Figures & data
Figure 1 Altered expression of Lnc712 and miR-129-5p was observed in OS. Lnc712 (A) and miR-129-5p (B) expression in paired non-tumor and OS tissues collected from OS patients (n=58) was determined by RT-qPCR. Average values of three technical replicates were presented and compared. ***p<0.001.
Figure 2 Lnc712 and miR-129-5p were inversely correlated. Linear regression was performed to analyze the correlations between Lnc712 and miR-129-5p across OS tissues (A) and non-tumor tissues (B).
Figure 3 MiR-129-5p may target Lnc712 to downregulate it in OS tissues. The potential interaction between Lnc712 and miR-129-5p was predicted by IntaRNA 2.0 (A). To explore the interaction between Lnc712 and miR-129-5p, Lnc712 expression vector or miR-129-5p mimic was transfected into MG-63 and Hs 3.T cells. Transfections were confirmed by RT-qPCR (B). The effects of Lnc712 expression vector transfection on miR-129-5p (C) and the effects of miR-129-5p mimic transfection on Lnc712 (D) were also explored by RT-qPCR. Data of three biological replicates was expressed as mean±SD. *p<0.05.
Figure 4 MiR-129-5p targeted Lnc712 to suppress cell proliferation. The roles of Lnc712 and miR-129-5p in regulating the proliferation of MG-63 and Hs 3.T cells were explored by cell proliferation assay. Data of three biological replicates was expressed as mean±SD. *p<0.05.
