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Review

Fourier Transform Infrared Spectroscopy: An Innovative Method for the Diagnosis of Ovarian Cancer

, , , , &
Pages 2389-2399 | Published online: 12 Mar 2021

Figures & data

Figure 1 Schematic illustration for the FTIR spectroscopy measurement flow. The main procedures include sample preparation, spectral acquisition and data analysis. Pretreatment of the samples depends on the sample type. The sample is then placed on a substrate, such as BaF2 slides, for IR spectroscopy. The representative IR spectrum is presented from an A2780 ovarian cancer cell line. The main absorption bands indicating cellular components are the asymmetric and symmetric vibrations (νasym CH3 and CH2) of fatty acyl moieties, ester C=O stretching of phospholipids, protein absorption bands (Amide I and Amide II), asymmetric and symmetric phosphodiester vibrations of nucleic acids (νasPO2 and νsPO2), and C–O–C vibrations for sugars. Spectral features were extracted and the data analysis was complex according to the aim of the research. The schematic refers to previous studies.Citation15Citation17

Figure 1 Schematic illustration for the FTIR spectroscopy measurement flow. The main procedures include sample preparation, spectral acquisition and data analysis. Pretreatment of the samples depends on the sample type. The sample is then placed on a substrate, such as BaF2 slides, for IR spectroscopy. The representative IR spectrum is presented from an A2780 ovarian cancer cell line. The main absorption bands indicating cellular components are the asymmetric and symmetric vibrations (νasym CH3 and CH2) of fatty acyl moieties, ester C=O stretching of phospholipids, protein absorption bands (Amide I and Amide II), asymmetric and symmetric phosphodiester vibrations of nucleic acids (νasPO2− and νsPO2−), and C–O–C vibrations for sugars. Spectral features were extracted and the data analysis was complex according to the aim of the research. The schematic refers to previous studies.Citation15–Citation17

Table 1 Diagnosis of Ovarian Cancer Using Tissue Samples Included in the Review

Table 2 Examples of Applications of FTIR Spectroscopy in Other Areas