Investigation of Chromosome 1 Aberrations in the Lymphocytes of Prostate Cancer and Benign Prostatic Hyperplasia Patients by Fluorescence in situ Hybridization

Figures & data

Figure 1 A representative example of a metaphase spread with normal chromosome 1 (A), translocation (B), acentric fragment (C), deletion (D), and insertion (E) detected by fluorescence in situ hybridization (FISH) using whole chromosome paints.

Table 1 The Mean Cellular Biomarkers Evaluated for PCa and BPH Patient Cells Irradiated with X-Rays

Group	MetAn	% AB.C(1)	T(1)F	Face(1)F	Del(1)F	Ins(1)F
PCa	15067	5.09	3.33	2.29	0.54	0.17
± S.D.	–	1.44	1.00	0.96	0.42	0.21
BPH	11487	2.70	2.08	0.48	0.44	0.04
± S.D.	–	1.21	0.95	0.27	0.42	0.10
p<	–	0.001*	0.001*	0.001*	ns**	0.001**

Notes: *Student’s t-test. **Mann–Whitney test. P values of ≤0.05 were considered to be significant and are bolded.

Abbreviations: MetAn, the total number of metaphases analyzed; % AB.C₍₁₎, the percentage of cells with aberrant chromosome 1; T₍₁₎F, F_ace(1)F, Del₍₁₎F, Ins₍₁₎F, the translocations, acentric fragments, deletions, and insertion frequencies of chromosome 1 per 100 metaphases/1 Gy, respectively.

Table 2 The Mean Frequency of Endogenous and Exogenous Chromosome 1 Aberrations Found in vivo, Evaluated in Non-Irradiated Cells from PCa and BPH Patients

Group	MetAn	% AB.C(1)	T(1)F	Face(1)F	Del(1)F	Ins(1)F
PCa	41563	0.39	0.27	0.06	0.04	0.04
± S.D.	–	0.14	0.13	0.06	0.04	0.07
BPH	27382	0.26	0.23	0.02	0.01	0.01
± S.D.	–	0.24	0.20	0.05	0.02	0.03
p<	–	0.05*	ns*	0.05*	0.05*	0.05*

Notes: *Mann–Whitney test. P values of ≤0.05 were considered to be significant and are bolded.

Abbreviations: MetAn, number of metaphases analyzed; % AB.C(1), the percentage of cells with aberrant chromosome 1; T(1)F, Face(1)F, Del(1)F, Ins(1)F, the translocation, acentric fragment, deletion, and insertion frequencies of chromosome 1 per 100 metaphases, respectively.

Table 3 Comparison of Cellular Radiosensitivity of Chromosome 1 Aberrations Obtained for PCa and BPH Patients, After Stratification by Cancer in the Immediate Family

	% AB.C(1) ±SD	T(1)F ± SD	Face(1)F ± SD	Del(1)F ± SD	Ins(1)F ± SD
PCa(CIF-)	4.18 ± 1.59	2.77 ± 1.08	1.85 ± 1.07	0.29 ± 0.31	0.15 ± 0.15
PCa(CIF+)	5.41 ± 1.61	3.41 ± 0.97	2.73 ± 1.12	0.77 ± 0.57	0.27 ± 0.28
BPH(CIF-)	2.41 ± 1.22	1.81 ± 0.97	0.44 ± 0.23	0.45 ± 0.47	0.05 ± 0.11
BPH(CIF+)	2.59 ± 1.01	1.97 ± 0.89	0.47 ± 0.25	0.40 ± 0.31	0.05 ± 0.09

Abbreviations: CIF, cancer incidence in the immediate family; % AB.C₍₁₎, the percentage of cells with aberrant chromosome 1; T₍₁₎F, F_ace(1)F, Del₍₁₎F, Ins₍₁₎F, the translocation, acentric fragment, deletion, and insertion frequencies of chromosome 1 per 100 metaphases/1 Gy.

Figure 2 The individual radiosensitivity (RS) presented as the frequency of acentric fragments (F_ace(1)F) of each PCa patient. The RS value is calculated for each donor. The central cross-line (the x-axis) represents the mean RS value and how the RS value varies among the donors. The lines (above and below the x-axis) showed on the histogram are the standard deviation (±SD) of the mean RS value. The patient identification number is typed on a single bar.