Figures & data
Table 1 The Correlations Between Clinicopathological Variables and the Expression of circRNA SCARB1 in HCC
Figure 1 The expression of circRNA SCARB1, mature miR-497 and miR-497 precursor was altered in HCC. HCC and paired non-tumor tissues were collected from the 64 HCC patients included in this study, and the expression of circRNA SCARB1 (A), mature miR-497 (B) and miR-497 precursor (C) in these tissue samples was determined by RT-qPCR. Average values of three technical replicates were used to express gene expression data in paired HCC and non-tumor tissues, **p < 0.01.
Figure 2 CircRNA SCARB1 and mature miR-497 were inversely correlated across HCC samples. Pearson’s correlation coefficient analysis was used to analyze the correlations between circRNA SCARB1 and mature miR-497 (A) or miR-497 precursor (B) across HCC tissue samples.
Figure 3 Overexpression of circRNA SCARB1 downregulated mature miR-497 in HCC cells. To explore the effects of overexpression of circRNA SCARB1 on the maturation of miR-497, SNU-423 and SNU-387 cells were transfected with either circRNA SCARB1 expression vector or miR-497 mimic, followed by the confirmation of transfections at 48 h post-transfection by RT-qPCR (A). The effects of overexpression of circRNA SCARB1 of the expression of mature miR-497 (B) and miR-497 precursor (C), as well as the effects of the transfection of miR-497 mimic on circRNA SCARB1 expression (D) were also analyzed by RT-qPCR. Mean ± SD values were used to express data of 3 biological replicates of in vitro cell experiments. *p < 0.05.
Figure 4 Overexpression of circRNA SCARB1 increased HCC cell proliferation through miR-497. CCK-8 assay was performed to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the proliferation of SNU-423 (A) and SNU-387 cells (B). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. *p < 0.05.
Figure 5 Overexpression of circRNA SCARB1 increased HCC cell migration through miR-497. Transwell assay was conducted to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the migration of SNU-423 (A and C) and SNU-387 cells (B and D). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. *p < 0.05.
