Transcriptome Signature of Immune Cells Post Reovirus Treatment in KRAS Mutated Colorectal Cancer

Figures & data

Table 1 Pathway Sources

	Pathway	Reference
1	Apoptosis Overview	Zambon A, Pico A, et al: Apoptosis Overview (Homo Sapiens). https://www.wikipathways.org/index.php/Pathway:WP254
2	Angiogenesis Overview	Helix, Willighagen E, et al: Angiogenesis Overview (Homo Sapiens). https://www.wikipathways.org/index.php/Pathway:WP1993
3	miR-targeted Genes of Lymphocytes	Sklar S, Hanspers, miR-targeted Genes in Lymphocytes (Homo Sapiens). https://www.wikipathways.org/index.php/Pathway:WP2004
4	Ras Signaling	Hanspers K, Slenter D, et al: Ras Signaling (Homo Sapiens). https://www.wikipathways.org/index.php/Pathway:WP4223
5	MAPK Signaling Pathway	Burel S, Hanspers K, et al: MAPK Signaling Pathway (Homo Sapiens). https://www.wikipathways.org/index.php/Pathway:WP382
6	EGF/EGFR Signaling Pathway	Pandey A, Hanspers, K, et al: EGF/EGFR Signaling Pathway (Homo Sapiens). https://www.wikipathways.org/index.php/Pathway:WP437
7	PI3K-Akt Signaling Pathway	Hanspers K, Riutta A, et al: PI3K-Akt Signaling Pathway (Homo Sapiens). https://www.wikipathways.org/index.php/Pathway:WP4172

Table 2 Genes Analyzed

	Gene Name	Gene Symbol
1	Son of Sevenless Homologue 1	SOS1
2	Ras-related Protein	RRAS
3	Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform	PIK3CB
4	MiR-16 microRNA precursor	MIR16-2
5	Cysteine and Histidine-rich Domain	CHORDC1
6	Reticulon-4	RTN4
7	Family with Sequence Similarity 96, Member A	FAM96A
8	Nuclear Factor Kappa-light-chain-enhancer of Activated B Cells	NFKB1, NFKBIA
9	Caspase-8	CASP8
10	Caspase-9	CASP9
11	Nitric Oxide Synthase 3	NOS3
12	Synaptic Nuclear Envelope Protein 1	SYNE1
13	Angiopoietin 1	ANGPT1
14	Vascular Endothelial Growth Factor	VEGF
15	Jun proto-oncogene	JUN
16	Insulin-like Growth Factor	IGF2

Figure 1 (A) The expression of SOS1 significant at 48 hr (B) The expression of RRAS significant at 48 hr (C) The expression of NFKB significant at D8 and D15.

Figure 2 (A) The expression of VEGFB significant at all timepoints (B) The expression of ANGPT1 significant at D8 (C) The expression of SYNE1 significant at D8 and D15.

Figure 3 (A) The expression of CASP8 significant at D8 and D15 (B) The expression of CASP9 significant at D8 (C) The expression of JUN significant at D15 (D) The expression of IGF2 significant at D8.

Figure 4 (A) The expression of CHORDC1 significant at 48hr and D15 (B) The expression of MIR16-2 significant at D15 (C) The expression of RTN4 significant at 48hr (D) The expression of FAM96A significant at 48hr.

Figure 5 (A) The expression of SOS1 significant at 48 hr (B) The expression of RRAS significant at 48 hr (C) The expression of PIK3CB significant at D8 and D15 (D) The expression of NOS3 significant at D15.

Figure 6 (A) Venn Diagram exhibiting pathway relationships between the primary 16 genes (B) STRING interaction network (GDE1 is an alias for MIR16-2). *Indicates a subunit of NF-κB. (C) Heat Map with fold changes from all 16 genes (D) Dendrogram showing statistical similarities between the 16 genes.