Prognostic Significance of CCNB2 Expression in Triple-Negative Breast Cancer

Figures & data

Table 1 Statistics of the Three Microarray Datasets Selected from the Gene Expression Omnibus Database

Dataset ID	Triple-Negative Breast Cancer	Normal	Total
GSE64790	3	3	6
GSE62931	47	53	100
GSE38959	30	13	43

Figure 1 Venn diagrams of DEGs common to three Gene Expression Omnibus datasets. (A) Total DEGs; (B) upregulated DEGs; (C) downregulated DEGs.

Abbreviation: DEG, differentially expressed gene.

Table 2 Significantly Enriched GO Terms and KEGG Pathways

Category	Term	Description	Count	p-value
BP	GO:0007067	Mitotic nuclear division	10	1.4E−7
BP	GO:0008283	Cell proliferation	10	3.6E−6
CC	GO:0005634	Nucleus	32	4.3E−4
CC	GO:0005654	Nucleoplasm	20	1.4E−3
MF	GO:0005515	Protein binding	42	4.8E−3
KEGG pathway	hsa04914	Progesterone-mediated oocyte maturation	4	3.7E−3
KEGG pathway	hsa04114	Oocyte meiosis	4	7.3E−3
KEGG pathway	hsa04110	Cell cycle	4	9.8E−3

Abbreviations: BP, biological process; CC, cellular component; MF, molecular function; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Figure 2 Enrichment analysis of DEGs and neighboring genes in triple-negative breast cancer. (A) Heatmap of enriched GO and KEGG terms, colored based on p-values. (B) Network of enriched GO and KEGG terms, colored based on p-values (terms containing more genes tend to have a more significant p-value). (C) PPI network. (D) Five most significant MCODE components from the PPI network. (E) DisGeNET data for the DEGs.

Abbreviations: DEG, differentially expressed gene; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; PPI, protein–protein interaction.

Figure 3 Transcription levels of the core genes in different types of cancers in Oncomine database (blue: low expression, red: high expression, comparison within the same line).

Figure 4 Expressions of the core genes in different breast cancer research microarrays. (A) CENPF expression in TCGA breast (1: breast, 2: invasive lobular breast carcinoma). (B) SKA1 expression in Turashvili breast (1: ductal breast cell, 2: lobular breast cell, 3: invasive ductal breast carcinoma). (C) BIRC5 expression in Curtis breast (1: breast, 2: medullary breast carcinoma). (D) CENPA expression in Curtis breast (1: breast, 2: invasive ductal breast carcinoma). (E) CCNB2 expression in Turashvili breast (1: ductal breast cell, 2: lobular breast cell, 3: invasive ductal breast carcinoma).

Figure 5 Prognostic values of mRNA expression levels of the core genes in patients with triple-negative breast cancer (Kaplan–Meier analysis). (A) Association of BIRC5 with RFS in TNBC. (B) Association of CENPA with RFS in TNBC. (C) Association of SKA1 with RFS in TNBC. (D) Association of CENPF with RFS in TNBC. (E) Association of CCNB2 with RFS in TNBC.

Abbreviations: RFS, relapse-free survival; TNBC, triple-negative breast cancer.

Figure 6 Immunohistochemical staining (EnVision Method). (A) CCNB2 is negatively expressed in the adjacent nontumor tissue at ×400 magnification. (B) CCNB2 is positively expressed in the triple-negative breast cancer tissue (magnification, ×400).

Table 3 Clinicopathological Characteristics and Kaplan–Meier Univariate Overall Survival Analysis of Patients with Triple-Negative Breast Cancer

Characteristic	Subgroup	Number of Samples	95% CI Lower Bound	95% CI Upper Bound	χ^2*	p
Age (years)	≤ 50	26	61.697	79.679	1.883	0.170
	> 50	13	46.244	69.827
Tumor size (cm)	≤ 2	17	45.493	74.502	2.140	0.143
	> 2	22	63.207	76.322
Location	Left	22	54.224	75.853	0.478	0.489
	Right	17	56.994	77.265
T	T1	17	45.493	74.502	6.681	0.035
	T2	20	68.256	76.744
	T3	2	26.000	26.000
N	N0	24	59.740	75.413	1.780	0.619
	N1	8	37.780	68.554
	N2	4	63.859	83.474
	N3	3	30.456	41.544
M	M0	35	59.427	75.784	0.104	0.747
	M1	4	40.792	63.708
CCNB2	Positive	21	45.131	68.858	5.265	0.022
	Negative	18	71.377	80.909

Abbreviations: CI, confidence interval; *χ², Log rank test chi-square value; CCNB2, cyclin B2.

Figure 7 Kaplan–Meier overall survival curves according to the (A) patient age (p = 0.170), (B) tumor size (p = 0.143), and (C) CCNB2 expression (p = 0.022).