CircRNA6783 Inhibits Cell Proliferation and Promotes Apoptosis in Lung Adenocarcinoma

Figures & data

Table 1 Pathological Characteristics of Study Population (n=70)

Pathological Characteristics	LA (n=40)	Normal (n=30)	P-value
Age (y)
<50	9	10	0.313
≥50	31	20
Gender
Male	21	17	0.448
Female	19	13
Smoking			0.938
Yes	11	8
No	29	22
TNM stage			-
I	7
II–III	15
IV	18

Abbreviations: LA, lung adenocarcinoma; TNM, tumor-node-metastasis.

Table 2 Primer Sequences

Primer	Primer Sequence
circRNA6783 -F	5’-TGTCCTGCAATTAGGTATCCGAAT-3’
circRNA6783 -R	5’-CTCTGGTTTATTTGTGGGGAAAGC-3’
cel-miR-39-5p-R	5’-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTATTAC-3’
cel-miR-39-5p-F	5’-TGGGAGCTGATTTCGTCTTG-3’

Abbreviations: F, forward; R, reverse.

Table 3 The Levels of CircRNA6783 in Patients with Lung Adenocarcinoma

Parameters	LA (n=40)	Normal (n=3.0)	t	P-value
circRNA6783 (relative expression)	0.65 ± 0.58	0.17 ± 0.14	4.414	< 0.001

Abbreviation: LA, lung adenocarcinoma.

Figure 1 The circRNA6783 levels were up-regulated in LA PWB and LA cells . (A) CircRNA6783 expression was detected using RT-PCR in cell lines. ***P<0.001 vs. Control. (B) The circRNA6783 expression was assessed in LA PWB. *P<0.05 vs. control.

Abbreviations: LA, lung adenocarcinoma; PWB, peripheral whole blood.

Figure 2 The ROC curve of circRNA6783. The area under the ROC curve (AUC) was to 0.830, 95% confidence interval (CI) = 0.731-0.929, P<0.001.

Figure 3 Overexpression of circRNA6783 (NCI-H1975 H17806) inhibited LA cell proliferation in vitro.***P<0.001 vs blank cells (NCI-H1975) and control cells (NCI-H1975 GL109). The values were the average of six independent experiments. ***P<0.001.

Abbreviation: CCK8, Cell Counting Kit-8.

Figure 4 Overexpression of circRNA6783 (NCI-H1975 H17806) promoted LA cell apoptosis in vitro. (A and B) The apoptosis rates of LA cells (Q2+Q3) were measured by flow cytometry 24 h after transfection with circRNA6783. Q1: dead cells; Q2: later apoptosis; Q3: early apoptosis; Q4: living cells. (C) NCI-H1975 H17806 promoted LA cell apoptosis in vitro. The values were the average of three independent experiments. ***P<0.001 vs. control (NCI-H1975 GL109).

Abbreviation: LA, lung adenocarcinoma.