Exploring the Expression and Prognosis of Mismatch Repair Proteins and PD-L1 in Colorectal Cancer in a Chinese Cohort

Figures & data

Figure 1 Expression of MSH2, MSH6, MLH1 and PMS2 proteins in CRC resection samples (IHC, ×400).

Notes: (A–D) show negative expression levels of MSH2, MSH6, MLH1, and PMS2, respectively; (E–H) show positive expression levels of MSH2, MSH6, MLH1, and PMS2, respectively.

Figure 2 PD-L1 expression in CRC resection samples (IHC, ×400).

Notes: (A and B) show negative and positive expression of PD-L1, respectively.

Table 1 Chi-Square Test Between the Expression of MMR and PD-L1 and Clinicopathological Features of CRC Primary Lesions

Pathological Parameters	MMR		X^2	P-value	PD-L1 Positive	X^2	P-value
	pMMR	dMMR
Age
<60	94	9	3.305	0.069	53	0.037	0.847
≥60	163	6			89
Gender
Male	167	11	0.437	0.508	94	0.075	0.784
Female	90	4			48
Tumor Location
Left	74	8	5.568	0.048*	21	1.877	0.405
Right	145	4			83
Rectum	38	3			38
Tumor diameter (cm)
≤5	206	11	0.409	0.523	29	0.008	0.931
>5	51	4			113
Nerve Violation
No	164	12	1.626	0.202	87	1.538	0.215
Yes	93	3			55
Vascular invasion
No	190	12	0.273	0.601	106	0.023	0.880
Yes	67	3			36
Lymph node metastasis
No	143	11	1.806	0.179	84	0.779	0.348
Yes	114	4			58
Type of pathology
Adenocarcinoma	255	14	4.505	0.157	142	3.313	0.108
Mucinous adenocarcinoma	2	1			0
Degree of differentiation
High	14	0	6.460	0.027*	36	0.554	0.780
Medium	187	7			7
Low	56	8			99
TNM Staging
I+II	114	3	7.241	0.007*	74	4.005	0.045*
III+IV	143	12			98

Abbreviations: X², X-squared; *Statistically significant P-values (P < 0.05).

Table 2 Chi-Square Test Between MLH1 Expression and PMS2 Expression

MLH1	PMS2		X^2	P-value
	-	+
-	7	0
+	3	262	188.245	<0.05
Total	10	262

Abbreviation: X², X-squared.

Table 3 Chi-Square Test Between MSH2 Expression and MSH6 Expression

MSH2	MSH6		X^2	P-value
	-	+
-	3	2
+	1	266	120.426	<0.05
Total	4	268

Abbreviation: X², X-squared.

Table 4 Chi-Square Test Between MMR Protein Expression and PD-L1 Expression

MMR Protein	PD-L1		Statistical Quantities	P-value
	-	+
MLH1
-	1	6	3.233	0.123
+	129	136
MSH2
-	1	4	1.577	0.373
+	129	138
MSH6
-	1	3	0.845	0.624
+	129	139
PMS2
-	1	9	5.943	0.020*
+	129	133

Abbreviation: *Statistically significant P-values (P < 0.05).

Figure 3 Log rank test between CRC patients in the dMMR group and those in the pMMR group.