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International Journal of Chronic Obstructive Pulmonary Disease Volume 14, 2019 - Issue
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Original Research

Reproducibility of fluid-phase measurements in PBS-treated sputum supernatant of healthy and stable COPD subjects

Fengyan Wang1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Zhenyu Liang1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Yuqiong Yang1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Luqian Zhou1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Lili Guan1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Weiliang Wu1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Mei Jiang1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Weijuan Shi1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Kuimiao Deng1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]
,
Jianhua Chen2 Respiratory Medicine Department, Guangzhou Panyu Central Hospital, Guangzhou 511400, People’s Republic of China
&
Rongchang Chen1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, People’s Republic of China, [email protected]Correspondence[email protected]
 show all
Pages 835-852 | Published online: 11 Apr 2019

Figures & data

Table 1 Demographic and clinical characteristics of subjects

Table 2 Paired-samples test results

Figure 1 Bland–Altman plots of soluble protein markers in sputum supernatant. Bland–Altman plots of (A) IL1β; (B) IL5; (C) IL6; (D) IL8; (E) IL10; (F) IL13; (G) IL33; (H) sRAGE; (I) TNFα; and (J) VEGF.

Abbreviations: IL, interleukin; sRAGE, soluble receptor for advanced glycation end-products; TNFα, tumor necrosis factor-α; VEGF, vascular endothelial growth factor.
Figure 1 Bland–Altman plots of soluble protein markers in sputum supernatant. Bland–Altman plots of (A) IL1β; (B) IL5; (C) IL6; (D) IL8; (E) IL10; (F) IL13; (G) IL33; (H) sRAGE; (I) TNFα; and (J) VEGF.

Table 3 Intraclass correlation coefficient (ICC) assessment results

Figure 2 Bland–Altman plots of IgG autoantibodies in sputum supernatant. Bland–Altman plots of (A) anti-aggrecan IgG; (B) anti-amyloid IgG; (C) anti-CENP-B IgG; (D) anti-collagen I IgG; (E) anti-collagen II IgG; (F) anti-collagen IV IgG; (G) anti-cytochrome C IgG; (H) anti-cytokeratin 18 IgG; (I) anti-elastin IgG; (J) anti-HSP47 IgG; (K) anti-HSP70 IgG; (L) anti-SS-B IgG; and (M) anti-vimentin IgG.

Abbreviations: CENP-B, centromere protein B; HSP47, heat shock protein 47; HSP70, heat shock protein 70; SS-B, La/Sjögren syndrome type B antigen.
Figure 2 Bland–Altman plots of IgG autoantibodies in sputum supernatant. Bland–Altman plots of (A) anti-aggrecan IgG; (B) anti-amyloid IgG; (C) anti-CENP-B IgG; (D) anti-collagen I IgG; (E) anti-collagen II IgG; (F) anti-collagen IV IgG; (G) anti-cytochrome C IgG; (H) anti-cytokeratin 18 IgG; (I) anti-elastin IgG; (J) anti-HSP47 IgG; (K) anti-HSP70 IgG; (L) anti-SS-B IgG; and (M) anti-vimentin IgG.
Figure 2 Bland–Altman plots of IgG autoantibodies in sputum supernatant. Bland–Altman plots of (A) anti-aggrecan IgG; (B) anti-amyloid IgG; (C) anti-CENP-B IgG; (D) anti-collagen I IgG; (E) anti-collagen II IgG; (F) anti-collagen IV IgG; (G) anti-cytochrome C IgG; (H) anti-cytokeratin 18 IgG; (I) anti-elastin IgG; (J) anti-HSP47 IgG; (K) anti-HSP70 IgG; (L) anti-SS-B IgG; and (M) anti-vimentin IgG.

Figure 3 Bland–Altman plots of IgM autoantibodies in sputum supernatant. Bland–Altman plots of (A) anti-aggrecan IgM; (B) anti-amyloid IgM; (C) anti-CENP-B IgM; (D) anti-collagen I IgM; (E) anti-collagen II IgM; (F) anti-collagen IV IgM; (G) anti-cytochrome C IgM; (H) anti-cytokeratin 18 IgM; (I) anti-elastin IgM; (J) anti-HSP47 IgM; (K) anti-HSP70 IgM; (L) anti-SS-B IgM; and (M) anti-vimentin IgM.

Abbreviations: CENP-B, centromere protein B; HSP47, heat shock protein 47; HSP70, heat shock protein 70; SS-B, La/Sjögren syndrome type B antigen.
Figure 3 Bland–Altman plots of IgM autoantibodies in sputum supernatant. Bland–Altman plots of (A) anti-aggrecan IgM; (B) anti-amyloid IgM; (C) anti-CENP-B IgM; (D) anti-collagen I IgM; (E) anti-collagen II IgM; (F) anti-collagen IV IgM; (G) anti-cytochrome C IgM; (H) anti-cytokeratin 18 IgM; (I) anti-elastin IgM; (J) anti-HSP47 IgM; (K) anti-HSP70 IgM; (L) anti-SS-B IgM; and (M) anti-vimentin IgM.
Figure 3 Bland–Altman plots of IgM autoantibodies in sputum supernatant. Bland–Altman plots of (A) anti-aggrecan IgM; (B) anti-amyloid IgM; (C) anti-CENP-B IgM; (D) anti-collagen I IgM; (E) anti-collagen II IgM; (F) anti-collagen IV IgM; (G) anti-cytochrome C IgM; (H) anti-cytokeratin 18 IgM; (I) anti-elastin IgM; (J) anti-HSP47 IgM; (K) anti-HSP70 IgM; (L) anti-SS-B IgM; and (M) anti-vimentin IgM.

Figure 4 Bland–Altman plots of sputum cell counts. Bland–Altman plots of (A) sputum neutrophils; (B) sputum macrophages; (C) sputum eosinophils; and (D) sputum lymphocytes.

Figure 4 Bland–Altman plots of sputum cell counts. Bland–Altman plots of (A) sputum neutrophils; (B) sputum macrophages; (C) sputum eosinophils; and (D) sputum lymphocytes.

Figure S1 Scatter plots of soluble protein markers with pair-wise comparisons of the two measurements. Scatter plots of (A) IL1β; (B) IL5; (C) IL6; (D) IL8; (E) IL10; (F) IL13; (G) IL33; (H) sRAGE; (I) TNFα; and (J) VEGF.

Abbreviations: IL, interleukin; sRAGE, soluble receptor for advanced glycation end-products; TNFα, tumor necrosis factor-α; VEGF, vascular endothelial growth factor.

Figure S1 Scatter plots of soluble protein markers with pair-wise comparisons of the two measurements. Scatter plots of (A) IL1β; (B) IL5; (C) IL6; (D) IL8; (E) IL10; (F) IL13; (G) IL33; (H) sRAGE; (I) TNFα; and (J) VEGF.Abbreviations: IL, interleukin; sRAGE, soluble receptor for advanced glycation end-products; TNFα, tumor necrosis factor-α; VEGF, vascular endothelial growth factor.

Figure S2 Scatter plots of IgG autoantibodies with pair-wise comparisons of the two measurements. Scatter plots of (A) anti-aggrecan IgG; (B) anti-amyloid IgG; (C) anti-CENP-B IgG; (D) anti-collagen I IgG; (E) anti-collagen II IgG; (F) anti-collagen IV IgG; (G) anti-cytochrome C IgG; (H) anti-cytokeratin 18 IgG; (I) anti-elastin IgG; (J) anti-HSP47 IgG; (K) anti-HSP70 IgG; (L) anti-SS-B IgG; and (M) anti-vimentin IgG.

Abbreviations: CENP-B, centromere protein B; HSP47, heat shock protein 47; HSP70, heat shock protein 70; SS-B, La/Sjögren syndrome type B antigen.

Figure S2 Scatter plots of IgG autoantibodies with pair-wise comparisons of the two measurements. Scatter plots of (A) anti-aggrecan IgG; (B) anti-amyloid IgG; (C) anti-CENP-B IgG; (D) anti-collagen I IgG; (E) anti-collagen II IgG; (F) anti-collagen IV IgG; (G) anti-cytochrome C IgG; (H) anti-cytokeratin 18 IgG; (I) anti-elastin IgG; (J) anti-HSP47 IgG; (K) anti-HSP70 IgG; (L) anti-SS-B IgG; and (M) anti-vimentin IgG.Abbreviations: CENP-B, centromere protein B; HSP47, heat shock protein 47; HSP70, heat shock protein 70; SS-B, La/Sjögren syndrome type B antigen.

Figure S3 Scatter plots of IgM autoantibodies with pair-wise comparisons of the two measurements. Scatter plots of (A) anti-aggrecan IgM; (B) anti-amyloid IgM; (C) anti-CENP-B IgM; (D) anti-collagen I IgM; (E) anti-collagen II IgM; (F) anti-collagen IV IgM; (G) anti-cytochrome C IgM; (H) anti-cytokeratin 18 IgM; (I) anti-elastin IgM; (J) anti-HSP47 IgM; (K) anti-HSP70 IgM; (L) anti-SS-B IgM; (M) anti-vimentin IgM.

Abbreviations: CENP-B, centromere protein B; HSP47, heat shock protein 47; HSP70, heat shock protein 70; SS-B, La/Sjögren syndrome type B antigen.

Figure S3 Scatter plots of IgM autoantibodies with pair-wise comparisons of the two measurements. Scatter plots of (A) anti-aggrecan IgM; (B) anti-amyloid IgM; (C) anti-CENP-B IgM; (D) anti-collagen I IgM; (E) anti-collagen II IgM; (F) anti-collagen IV IgM; (G) anti-cytochrome C IgM; (H) anti-cytokeratin 18 IgM; (I) anti-elastin IgM; (J) anti-HSP47 IgM; (K) anti-HSP70 IgM; (L) anti-SS-B IgM; (M) anti-vimentin IgM.Abbreviations: CENP-B, centromere protein B; HSP47, heat shock protein 47; HSP70, heat shock protein 70; SS-B, La/Sjögren syndrome type B antigen.

Figure S4 Scatter plots of sputum cell counts with pair-wise comparisons of the two measurements. Scatter plots of (A) sputum neutrophils; (B) sputum macrophages; (C) sputum eosinophils; and (D) scatter plots of sputum lymphocytes.

Figure S4 Scatter plots of sputum cell counts with pair-wise comparisons of the two measurements. Scatter plots of (A) sputum neutrophils; (B) sputum macrophages; (C) sputum eosinophils; and (D) scatter plots of sputum lymphocytes.

Table S1 All measurement results

Table S2 Comparison of measurements between groups

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