Figures & data
Figure 1 BYF inhibits CSE-induced cellular senescence. The protein levels of senescence markers p16, p21 and p27 were detected by Western blot (A and B). The secretion of IL-6 (C), IL-8 (D), and CCL3 (E) into the medium was measured by ELISA. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; #Compared into CSE group, P<0.05; ##Compared into CSE group, P<0.01.
![Figure 1 BYF inhibits CSE-induced cellular senescence. The protein levels of senescence markers p16, p21 and p27 were detected by Western blot (A and B). The secretion of IL-6 (C), IL-8 (D), and CCL3 (E) into the medium was measured by ELISA. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; #Compared into CSE group, P<0.05; ##Compared into CSE group, P<0.01.](/cms/asset/573382ce-42ca-47e8-9ea0-b3dd2f9f24c7/dcop_a_12156782_f0001_c.jpg)
Figure 2 BYF up-regulates the transcription, expression and secretion of klotho. The transcription (A), expression (B and C) and secretion (D) of klotho in BEAS-2B cells were detected by RT-qPCR, Western blot and ELISA, respectively. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; ##Compared into CSE group, P<0.01; ###Compared into CSE group, P<0.001.
![Figure 2 BYF up-regulates the transcription, expression and secretion of klotho. The transcription (A), expression (B and C) and secretion (D) of klotho in BEAS-2B cells were detected by RT-qPCR, Western blot and ELISA, respectively. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; ##Compared into CSE group, P<0.01; ###Compared into CSE group, P<0.001.](/cms/asset/0e17e0db-196a-4ae0-b7a4-10e6ac5db357/dcop_a_12156782_f0002_c.jpg)
Figure 3 BYF activates klotho transcription by upregulating the expression of transcription factor ZNF263. ZNF263 binding klotho promoter sequence with JASPAR and USCS databases (A). The protein level of ZNF263 in BEAS-2B cells with CSE exposure and BYF treatment was detected by Western blot (B and C). The protein levels of ZNF263, klotho and senescence markers in BEAS-2B cells with CSE exposure and ZNF263-expression plasmid transfection were detected by Western blot (D–F). The secretion of IL-6 (G), IL-8 (H), and CCL3 (I) into the medium was measured by ELISA. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; #Compared into CSE group, P<0.05; ##Compared into CSE group, P<0.01; ###Compared into CSE group, P<0.001.
![Figure 3 BYF activates klotho transcription by upregulating the expression of transcription factor ZNF263. ZNF263 binding klotho promoter sequence with JASPAR and USCS databases (A). The protein level of ZNF263 in BEAS-2B cells with CSE exposure and BYF treatment was detected by Western blot (B and C). The protein levels of ZNF263, klotho and senescence markers in BEAS-2B cells with CSE exposure and ZNF263-expression plasmid transfection were detected by Western blot (D–F). The secretion of IL-6 (G), IL-8 (H), and CCL3 (I) into the medium was measured by ELISA. *Compared into NC group, P<0.05; **Compared into NC group, P<0.01; ***Compared into NC group, P<0.001; #Compared into CSE group, P<0.05; ##Compared into CSE group, P<0.01; ###Compared into CSE group, P<0.001.](/cms/asset/d57644d0-bf77-446f-84ea-45a935d16dc0/dcop_a_12156782_f0003_c.jpg)