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Drug Design, Development and Therapy Volume 12, 2018 - Issue
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Original Research

Tanshinol ameliorates CCl4-induced liver fibrosis in rats through the regulation of Nrf2/HO-1 and NF-κB/IκBα signaling pathway

Rong WangDepartment of Pharmacy, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
,
Jing WangDepartment of Pharmacy, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
,
Fuxing SongDepartment of Pharmacy, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
,
Shengnan LiDepartment of Pharmacy, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
&
Yongfang YuanDepartment of Pharmacy, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaCorrespondence[email protected]
Pages 1281-1292 | Published online: 17 May 2018

Figures & data

Table 1 Effect of tanshinol on body weight, liver weight and liver index of rats (mean±SD)

Table 2 Effect of tanshinol on serum concentrations of ALT, AST, and TBIL

Table 3 Effect of tanshinol on serum concentrations of HA, LN, IV-C, and PIIIP

Figure 1 Effect of tanshinol on the morphology and architecture of the liver.

Notes: (A) H&E staining. Magnification ×100. (B) Masson’s trichrome staining. Magnification ×100. (C) the scores of liver fibrosis degree. (D) The area of collagen fibers in liver (100%). ap<0.05, compared with the control group, bp<0.05, compared with the model group, cp<0.05, compared with the tanshinol 20 mg/kg group. The different mark represents liver fibrosis degree score of each rat in different group.

Figure 1 Effect of tanshinol on the morphology and architecture of the liver.Notes: (A) H&E staining. Magnification ×100. (B) Masson’s trichrome staining. Magnification ×100. (C) the scores of liver fibrosis degree. (D) The area of collagen fibers in liver (100%). ap<0.05, compared with the control group, bp<0.05, compared with the model group, cp<0.05, compared with the tanshinol 20 mg/kg group. The different mark represents liver fibrosis degree score of each rat in different group.

Figure 2 Effect of tanshinol on expression level of (A) SOD, (B) GSH-Px, and (C) MDA in CCl4-induced liver tissues.

Notes: ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.

Abbreviations: CCl4, carbon tetrachloride; GSH-Px, glutathione peroxidase; MDA, malondialdehyde; SOD, superoxide dismutase.

Figure 2 Effect of tanshinol on expression level of (A) SOD, (B) GSH-Px, and (C) MDA in CCl4-induced liver tissues.Notes: ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.Abbreviations: CCl4, carbon tetrachloride; GSH-Px, glutathione peroxidase; MDA, malondialdehyde; SOD, superoxide dismutase.

Figure 3 Effect of tanshinol on inflammatory factors (TGF-β, TNF-α, Cox-2, IL-1β, and IL-6) in CCl4-induced liver tissues.

Notes: ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.

Abbreviations: Cox-2, cyclooxygenase-2; IL, interleukin; TNF-α, tumor necrosis factor-α; TGF-β, transforming growth factor-β.

Figure 3 Effect of tanshinol on inflammatory factors (TGF-β, TNF-α, Cox-2, IL-1β, and IL-6) in CCl4-induced liver tissues.Notes: ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.Abbreviations: Cox-2, cyclooxygenase-2; IL, interleukin; TNF-α, tumor necrosis factor-α; TGF-β, transforming growth factor-β.

Figure 4 Effect of tanshinol on the expression of NF-κB signaling pathway.

Notes: (A) Effect of tanshinol on the expression of nuclear NF-κB by Western blot. (B) Effect of tanshinol on the expression of cytosolic NF-κB and IκBα by Western blot. ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.
Abbreviations: IκBα, inhibitor of kappa B; NF-κB, nuclear factor kappa B.
Figure 4 Effect of tanshinol on the expression of NF-κB signaling pathway.

Figure 5 Effect of tanshinol on expression of Nrf2 in cell nucleus (A) and cytoplasm (B) in CCl4-induced liver tissues.

Notes: ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.

Abbreviations: CCl4, carbon tetrachloride; Nrf2, nuclear factor erythroid2-related factor 2.

Figure 5 Effect of tanshinol on expression of Nrf2 in cell nucleus (A) and cytoplasm (B) in CCl4-induced liver tissues.Notes: ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.Abbreviations: CCl4, carbon tetrachloride; Nrf2, nuclear factor erythroid2-related factor 2.

Figure 6 Effect of tanshinol on expression of HO-1, NQO-1 and GCLC in CCl4-induced liver tissues.

Notes: Western blot, real-time polymerase chain reaction (A) and immunohistochemical staining. Magnification ×100. (B) were used to examine the expression level. ap<0.05, compared with the control group; bp<0.05, compared with the model group; cp<0.05, compared with the tanshinol 20 mg/kg group.
Abbreviations: CCl4, carbon tetrachloride; GCLC, glutamate-cysteine ligase; HO-1, hemeoxygenase-a; NQO-1, quinine oxidoreductase-1.
Figure 6 Effect of tanshinol on expression of HO-1, NQO-1 and GCLC in CCl4-induced liver tissues.

Table S1 Primer sequences for real-time polymerase chain reaction assay

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