Lobaplatin inhibits breast cancer progression, cell proliferation while it induces cell apoptosis by downregulating MTDH expression

Figures & data

Table 1 The differences in general data and complications within 72 hours after operations, and the adverse events during a 4-year follow-up

Items	Control	Treatment	P-value
General data before and within 72 hours after operations
Age (years)	46.4±5.7	47.1±6.3	>0.05
WBC (×10^9)	7.61±2.01	7.32±1.20	>0.05
PLT (×10^9)	229.40±74.27	227.12±45.37	>0.05
Creatinine (µmol/L)	76.81±5.44	75.43±6.37	>0.05
ALT (U/L)	33.67±4.76	33.18±6.01	>0.05
Drainage (mL)	572.15±47.23	504.81±60.48	<0.05
Healing time (days)	18.2±1.1	17.7±2.7	>0.05
Complications within 72 hours after operations
Pain	34.75% (11/32)	31.25% (10/32)	>0.05
Nausea	25.00% (8/32)	28.13% (9/32)	>0.05
Vomiting	12.50% (4/32)	12.50% (4/32)	>0.05
Diarrhea	6.25% (2/32)	3.13% (1/32)	>0.05
Patients with exfoliated cancer cells	12.50% (4/32)	31.25% (10/32)	<0.05
Adverse events during follow-up (4 years)
Recurrence	3.13% (1/32)	6.25% (2/32)	>0.05
Metastasis	0 (0/32)	3.13% (1/32)	>0.05

Note: Patients in treatment group were treated with lobaplatin after cleaning procedures, followed by radical mastectomies for BCa patients.

Abbreviations: ALT, alanine aminotransferase; BCa, breast cancer; PLT, platelet count; WBC, white cell count.

Figure 1 Lobaplatin induces MCF-7 cell apoptosis.

Notes: (A) Cell proliferation analysis. (Bi and ii) Cell apoptosis analysis using flow cytometric analysis. *, **, and *** indicate P<0.05, 0.01, and 0.001 vs control, respectively.

Figure 2 Lobaplatin affected the expression of MTDH and apoptosis-related protein in MCF-7 cells.

Notes: (A and B) The protein expression of Bcl-2 and Bax by Western blot analysis. (C) The Bcl-2/Bax ratio. **, and *** indicate P<0.05, 0.01, and 0.001 vs control, respectively.
Abbreviation: MTDH, metadherin.

Figure 3 The expression of MTDH in lobaplatin-treated cells.

Notes: The mRNA expression level (left) and protein fold change of MTDH (right), respectively, were detected using qRT-PCR and Western blot analysis. *, **, and *** indicate P<0.05, 0.01, and 0.001 vs control, respectively.
Abbreviations: MTDH, metadherin; qRT-PCR, quantitative reverse transcriptase-polymerase chain reaction.

Figure 4 Effect of MTDH inhibition on MCF-7 cell apoptosis and proliferation.

Notes: (A) The expression of MTDH protein by Western blot analysis. (B) Cell apoptosis was analyzed using flow cytometric analysis. (C and D) The expression of Bcl-2 and Bax proteins, respectively. (E) The Bcl-2/Bax ratio. *, **, and *** indicate P<0.05, 0.01, and 0.001 vs control, respectively. ^#, ^##, and ^### indicate differences at P<0.05, 0.01, and 0.001 vs 50 mg/L lobaplatin.
Abbreviation: MTDH, metadherin.