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Original Research

Predicting and Exploring the Mechanisms of Erzhi Pill in Prevention and Treatment of Osteoporosis Based on Network Pharmacology and Zebrafish Experiments

, , , , , & ORCID Icon show all
Pages 817-827 | Published online: 24 Feb 2021

Figures & data

Table 1 The Top 15 High-Degree Compounds for RZP in the Treatment of OP

Figure 1 Intersection of RZP targets and OP-related targets.

Figure 1 Intersection of RZP targets and OP-related targets.

Figure 2 The regulatory network of EZP in the treatment of OP. This network contained 72 compounds, 220 targets, 20 significant KEGG pathways and 2 Chinese herbal medicine.

Figure 2 The regulatory network of EZP in the treatment of OP. This network contained 72 compounds, 220 targets, 20 significant KEGG pathways and 2 Chinese herbal medicine.

Figure 3 The top 15 most significant KEGG pathways for EZP in the treatment of OP.

Figure 3 The top 15 most significant KEGG pathways for EZP in the treatment of OP.

Figure 4 The PI3K-Akt signaling pathway regulated by EZP.

Figure 4 The PI3K-Akt signaling pathway regulated by EZP.

Table 2 Protective Effects of QU Against Dex-Induced Inhibition of Skull Mineralization and Osteoblast Differentiation in AB Strain and Tg(sp7: egfp) Larval Zebrafish, Respectively (n ≥9)

Figure 5 QU counteracts Dex-induced decrease of skull mineralization in AB strain larval zebrafish. Dorsal view of skull stained by alizarin red in AB strain larval zebrafish at 9 dpf exposed to Dex (10μM) in the presence or absence of QU for 6 d.

Notes: Veh (vehicle control, 0.1% dimethyl sulphoxide); Dex (Dex 10μM); D+QU0.5 (Dex+ QU 0.5μM); D+QU1 (Dex+QU 1μM); D+QU2 (Dex+QU 2μM); D+QU4 (Dex+QU 4μM); D+QU8 (Dex+QU 8μM); D+QU16 (Dex+QU 16μM).
Figure 5 QU counteracts Dex-induced decrease of skull mineralization in AB strain larval zebrafish. Dorsal view of skull stained by alizarin red in AB strain larval zebrafish at 9 dpf exposed to Dex (10μM) in the presence or absence of QU for 6 d.

Figure 6 QU attenuated Dex-induced inhibition of skull osteoblasts differentiation in tg(sp7: egfp) larval zebrafish. Green fluorescence images of skull dorsal aspect using LSCM in tg(sp7: egfp) larval zebrafish at 9 dpf exposure to Dex (10μM) in the presence or absence of QU for 6 d.

Notes: Veh (vehicle control, 0.1% dimethyl sulphoxide); Dex (Dex 10μM); D+QU0.5 (Dex+ QU 0.5μM); D+QU1 (Dex+QU 1μM); D+QU2 (Dex+QU 2μM); D+QU4 (Dex+QU 4μM); D+QU8 (Dex+QU 8μM); D+QU16 (Dex+QU 16μM).
Figure 6 QU attenuated Dex-induced inhibition of skull osteoblasts differentiation in tg(sp7: egfp) larval zebrafish. Green fluorescence images of skull dorsal aspect using LSCM in tg(sp7: egfp) larval zebrafish at 9 dpf exposure to Dex (10μM) in the presence or absence of QU for 6 d.