Figures & data
Figure 1 Structural formula of Tet and a schematic of intravitreal treatment and partitions of the retina for RGC counting.
Abbreviations: RGC, retinal ganglion cell; Tet, tetrandrine.
![Figure 1 Structural formula of Tet and a schematic of intravitreal treatment and partitions of the retina for RGC counting.](/cms/asset/f5b12724-b173-4b39-a892-c33d3cca8894/dddt_a_55407_f0001_b.jpg)
Figure 2 Effects of Tet on the survival of RGC-5 cells and SSP-induced neuron-like cells under serum deprivation conditions.
Abbreviations: ANOVA, analysis of variance; DMSO, dimethyl sulfoxide; FBS, fetal bovine serum; SD, standard deviation; SSP, staurosporine; Tet, tetrandrine.
![Figure 2 Effects of Tet on the survival of RGC-5 cells and SSP-induced neuron-like cells under serum deprivation conditions.](/cms/asset/31490caf-781d-4291-9e8d-1ed58c72527b/dddt_a_55407_f0002_b.jpg)
Figure 3 Tet protected RGC-5 cells from H2O2 injury and L-glutamate excitotoxicity.
Abbreviations: ANOVA, analysis of variance; L-glu, L-glutamate; SD, standard deviation; Tet, tetrandrine.
![Figure 3 Tet protected RGC-5 cells from H2O2 injury and L-glutamate excitotoxicity.](/cms/asset/96035128-9677-4587-9ceb-958ecaf3b175/dddt_a_55407_f0003_b.jpg)
Figure 4 The identification, morphology, and survival curve of primary cultured RGCs.
Abbreviations: DAPI, 4, 6-diamidino-2-phenylindole; MAP2, microtubule associated protein 2; RGCs, retinal ganglion cells.
![Figure 4 The identification, morphology, and survival curve of primary cultured RGCs.](/cms/asset/9db7bced-83f7-4763-9ad7-1ceb513ba3d5/dddt_a_55407_f0004_c.jpg)
Figure 5 Time-dependent effect of Tet on the viability of primary cultured RGCs.
Abbreviations: ANOVA, analysis of variance; DMSO, dimethyl sulfoxide; RGCs, retinal ganglion cells; SD, standard deviation; Tet, tetrandrine.
![Figure 5 Time-dependent effect of Tet on the viability of primary cultured RGCs.](/cms/asset/49082b26-26bb-4b79-b9b0-e3367dbd1b68/dddt_a_55407_f0005_b.jpg)
Figure 6 Tet protects RGCs from ischemic injury in vivo.
Abbreviations: CNTF, ciliary neurotrophic factor; GCL, ganglion cell layers; I/R, ischemia/reperfusion; PBS, phosphate-buffered saline; RGCs, retinal ganglion cells; Tet, tetrandrine.
![Figure 6 Tet protects RGCs from ischemic injury in vivo.](/cms/asset/eef8f24b-f8c9-4fd8-a03d-a7c82fbf70bf/dddt_a_55407_f0006_c.jpg)
Figure 7 Statistical analysis of RGC survival following Tet application in vivo.
Abbreviations: ANOVA, analysis of variance; CNTF, ciliary neurotrophic factor; I/R, ischemia/reperfusion; PBS, phosphate-buffered saline; RGC, retinal ganglion cell; SD, standard deviation; Tet, tetrandrine.
![Figure 7 Statistical analysis of RGC survival following Tet application in vivo.](/cms/asset/aeb97341-e82a-4614-8556-35a9007d8485/dddt_a_55407_f0007_b.jpg)
Figure 8 Effects of Tet and CNTF on primary cultured RGCs ΔΨm.
Abbreviations: ΔΨm, inner mitochondrial membrane potential; CNTF, ciliary neurotrophic factor; FCCP, carbonyl cyanide 4-trifluoromethoxyphenylhydrazone; FITC, fluorescein isothiocyanate; LL, lower left; LR, lower right; PBS, phosphate-buffered saline; PE, P-phycoerythrin; RGCs, retinal ganglion cells; Tet, tetrandrine; UL, upper left; UR, upper right.
![Figure 8 Effects of Tet and CNTF on primary cultured RGCs ΔΨm.](/cms/asset/da491fbd-5b14-4137-8100-16978e4e4450/dddt_a_55407_f0008_b.jpg)
Figure 9 Tet and CNTF inhibited the expression of cleaved caspase-3 in the retinas 1 day after I/R insult.
Abbreviations: CNTF, ciliary neurotrophic factor; DAPI, 4, 6-diamidino-2-phenylindole; I/R, ischemia/reperfusion; PBS, phosphate-buffered saline; Tet, tetrandrine; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer.
![Figure 9 Tet and CNTF inhibited the expression of cleaved caspase-3 in the retinas 1 day after I/R insult.](/cms/asset/45ec0052-4955-4d14-b0cb-99a470feb139/dddt_a_55407_f0009_c.jpg)
Figure 10 Tet and CNTF inhibited Bcl-2 expression 1 day after I/R insult.
Abbreviations: CNTF, ciliary neurotrophic factor; DAPI, 4′, 6-diamidino-2-phenylindole; I/R, ischemia/reperfusion; PBS, phosphate-buffered saline; Tet, tetrandrine.
![Figure 10 Tet and CNTF inhibited Bcl-2 expression 1 day after I/R insult.](/cms/asset/178a0df0-a5ae-472b-8fcf-906843ad0d18/dddt_a_55407_f0010_c.jpg)