Figures & data
Table 1 Basic Clinical Characters of Participants in the 3 Groups at the First Diagnose
Table 2 Primer Sequences Used in PCR
Figure 1 LINC00673 and p53 showed opposite expression pattern in DR. LINC00673 and p53 levels in the plasma of 3 groups of participants were detected by RT-qPCR and ELISA. (A) The expressions of LINC00673 levels in DR group, DB group, and control groups were detected by RT-qPCR. (B) Plasma p53 levels in DR group, DB group, and Control groups were detected by the ELISA. The data were compared by ANOVA (one-way) and Tukey’s test. *p<0.05.
Figure 2 LINC00673 negatively regulated p53. Linear regression showed that LINC00673 and p53 were inversely and significantly correlated in plasma of DR patients (A). At 24h after the transfections of LINC00673 expression vector and siRNA, LINC00673 expression was significantly altered compared to NC and C groups, indicating the successful transfections (B). In addition, compared to NC and C groups, LINC00673 overexpression downregulated while LINC00673 siRNA silencing upregulated p53 at both mRNA and protein levels (C). *p<0.05.
Figure 3 High glucose environment downregulated LINC00673 in h1RPE7 and ARPE-19 cells. h1RPE7 cells (A) and ARPE-19 cells (B) were treated with D-glucose at doses of 5, 10, 30, and 60 mM for 24h, and LINC00673 expression was detected by RT-qPCR and analyzed by ANOVA (one-way) and Tukey’s test. It was observed that D-glucose downregulated LINC00673 in a concentration-dependent manner. *p<0.05.
Figure 4 LINC00673 inhibited apoptosis of h1RPE7 and ARPE-19 cells under a high glucose environment via p53. h1RPE7 cells (A) and ARPE-19 cells (B) were treated with 30 and 60 mM D-glucose for 24h, followed by the analysis of cell apoptosis. Compared to C and NC groups, LINC00673 overexpression decreased while LINC00673 siRNA silencing increased the apoptotic rate of RPECs. In addition, p53 overexpression reduced the effect of LINC00673 overexpression. *p<0.05.
