Associations of TCF7L2 rs11196218 (A/G) and GLP-1R rs761386 (C/T) Gene Polymorphisms with Obesity in Chinese Population

Figures & data

Table 1 Clinical and Biochemical Characteristics of the Studied Groups

Characteristics	Non-Obese (n=69)	Obese (n=60)	P value
Sex (F/M)	47/22	31/29	0.084
Age (year)	37.16±8.74	34.37±8.54	0.070
BMI (kg/m^2)	21.14±1.72	32.67±5.42	<0.001
WHR	0.79±0.05	0.95±0.06	<0.001
FBG (mmol/L)	5.06±0.29	5.08±0.37	0.783
SBP (mmHg)	110.22±10.94	120.30±20.28	<0.001
DBP (mmHg)	71.28±7.98	79.23±14.28	<0.001
TC (mmol/L)	4.44±0.49	5.06±0.82	<0.001
TG (mmol/L)	0.98±0.31	2.28±1.37	<0.001
HDL-C (mmol/L)	1.57±0.33	1.21±0.26	<0.001
LDL-C (mmol/L)	2.49±0.43	3.24±0.72	<0.001

Abbreviations: BMI, body mass index; WHR, waist-to-hip ratio; FPG, fasting plasma glucose; SBP, systemic blood pressure; DBP, diastolic blood pressure; TC, total cholesterol; TG, serum total triacylglycerol; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol.

Table 2 Hardy–Weinberg Equilibrium Test and Allelic Frequencies

SNP	Allele	Non-Obese (n=69)	Obese (n=60)	P value	OR	95% CI
rs11196218	A	26(0.188)	42(0.350)	0.003	2.32	1.31~4.09
	G	112(0.812)	78(0.650)
	Total (2n)	138	120
	HWE P	0.665	0.843
rs761386	C	117(0.848)	89(0.742)
	T	21(0.152)	31(0.258)	0.034	1.94	1.05~3.60
	Total (2n)	138	120
	HWE P	0.136	0.502

Abbreviations: SNP, single-nucleotide polymorphism; P, Pearson’s P value; HWE, Hardy–Weinberg equilibrium. The results are presented as n (%).

Table 3 Genotypic Frequencies Between Non-Obese and Obese Group

SNP	Genotype	Non-Obese (n=69)	Obese (n=60)	χ^2	P value
rs11196218	A/A	3(0.043)	7(0.117)	8.558	0.014
	A/G	20(0.290)	28(0.467)
	G/G	46(0.667)	25(0.417)
rs761386	C/C	48(0.696)	34(0.567)	6.795	0.033
	C/T	21(0.304)	21(0.350)
	T/T	0(0.000)	5(0.083)

Note: The results are presented as n (%).

Abbreviations: SNP, single-nucleotide polymorphism; P, Pearson’s P value.

Table 4 Binary Logistic Regression Analysis of Obesity and Genotype – Dominant Model

SNP	β	Seβ	P value	OR	95% CI
rs11196218
Age	0.029	0.022	0.185	1.03	0.99~1.08
Sex	−0.730	0.381	0.056	0.48	0.23~1.02
(AA/AG vs GG)	0.933	0.380	0.014	2.54	1.21~5.35
rs761386
Age	0.038	0.021	0.073	1.04	1.00~1.08
Sex	−0.702	0.374	0.061	0.50	0.24~1.03
(CT/TT vs CC)	0.478	0.379	0.208	1.61	0.77~3.39

Note: Age, sex and genotype are independent variables, group (obesity or non-obesity) is the binary dependent variable.

Abbreviation: SNP, single-nucleotide polymorphism.

Table 5 Binary Logistic Regression Analysis of Obesity and Genotype – Recessive Model

SNP	β	Seβ	P value	OR	95% CI
rs11196218
Age	0.039	0.021	0.070	1.04	1.00~1.08
Sex	0.821	0.380	0.031	2.27	1.08~4.78
(AA vs AG/GG)	1.233	0.742	0.097	3.43	0.80~14.71
rs761386
Age	0.047	0.022	0.035	1.048	1.00~1.10
Sex	0.685	0.382	0.073	1.984	0.94~4.19
(TT vs CT/CC)	21.554	17,509.348	0.999	2.295E9	0.00~.

Note: Age, sex and genotype are independent variables, group (obesity or non-obesity) is the binary dependent variable.

Abbreviation: SNP, single-nucleotide polymorphism.

Table 6 Comparison of Clinical and Biological Parameters Between Genotypes

	AA/AG (n= 58)	GG (n= 71)	P value	P-adjusted
rs11196218
BMI (kg/m^2)	27.56±7.38	25.64±6.52	0.120	0.267
WHR	0.88±0.10	0.85±0.93	0.034	0.082
FBG (mmol/L)	5.08±0.32	5.06±0.33	0.679	0.556
SBP (mmHg)	116.16±12.42	113.89±19.54	0.445	0.601
DBP (mmHg)	75.66±8.91	74.42±14.05	0.563	0.749
TC (mmol/L)	4.85±0.67	4.64±0.77	0.108	0.138
TG (mmol/L)	1.71±1.27	1.38±1.06	0.257	0.270
HDL-C (mmol/L)	1.35±0.32	1.44±0.37	0.148	0.199
LDL-C (mmol/L)	2.97±0.69	2.73±0.68	0.059	0.072
	CT/TT (n= 47)	CC (n= 82)	P value	P-adjusted
rs761386
BMI (kg/m^2)	27.14±6.31	26.14±7.32	0.436	0.616
WHR	0.88±0.10	0.86±0.10	0.322	0.466
FBG (mmol/L)	5.08±0.40	5.06±0.29	0.739	0.674
SBP (mmHg)	112.09±19.63	116.52±14.64	0.147	0.107
DBP (mmHg)	73.28±13.63	75.95±10.91	0.224	0.166
TC (mmol/L)	4.86±0.78	4.66±0.70	0.143	0.190
TG (mmol/L)	1.74±1.23	1.49±1.11	0.241	0.350
HDL-C (mmol/L)	1.34±0.33	1.43±0.36	0.181	0.289
LDL-C (mmol/L)	2.96±0.80	2.77±0.62	0.120	0.181

Note: P-adjusted, adjusted the confounder such as sex and age by multiple linear regression analysis.

Abbreviations: BMI, body mass index; WHR, waist-to-hip ratio; FPG, fasting plasma glucose; SBP, systemic blood pressure; DBP, diastolic blood pressure; TC, total cholesterol; TG, serum total triacylglycerol; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol.