The Single Nucleotide Polymorphisms (rs1292037 and rs13137) in miR-21 Were Associated with T2DM in a Chinese Population

Figures & data

Table 1 Clinical Characteristics and Glucose and Lipid Metabolic Parameters of the Subjects Enrolled in the Present Study (Data are the Mean ± SD)

	NDM	T2DM	P value
N	1656	1593
Age (years)	58.192±11.543	58.014±12.233	0.671
Sex (M/F)	1048/608	990/603	0.263
Age (M; years)	58.385±11.405	57.726±12.497	0.215
Age (F; years)	57.860±11.780	58.488±11.781	0.354
Total cholesterol (mmol/L)	4.897±1.020	4.817±1.118	0.037
Triglycerides (mmol/L)	1.712±1.149	2.458±2.085	<0.001
High-density lipoprotein-cholesterol (mmol/L)	1.339±0.355	1.085±0.284	<0.001
Low-density lipoprotein-cholesterol (mmol/L)	3.080±0.890	2.808±0.959	<0.001
Fasting plasma glucose (mmol/L)	5.156±0.487	8.136±2.624	<0.001
Glycosylated haemoglobin (%)	5.135±0.300	9.064±2.703	<0.001

Table 2 Comparison of Genotypic and Allelic Distribution of Eight SNPs Among Six microRNAs Between T2DM Group and NDM Group

SNPs	Alleles/Genotypes	T2DM n(Freq)	NDM n(Freq)	Chi^Citation2	P value	Odds Ratio[95% CI]
rs78312845	A	2748(0.863)	2907(0.878)	3.320	0.068	0.87 [0.76–1.01]
	G	438(0.137)	405(0.122)
	A/A	1183(0.743)	1276(0.771)	3.460	0.178
	A/G	382(0.240)	355(0.214)
	G/G	28(0.018)	25(0.015)
	H-W	0.656	0.957
rs2297537	C	2589(0.813)	2652(0.803)	0.936	0.333	1.06 [0.94–1.20]
	G	597(0.187)	650(0.197)
	C/C	1063(0.667)	1065(0.645)	2.568	0.277
	C/G	463(0.291)	522(0.316)
	G/G	67(0.042)	64(0.039)
	H-W	0.069	0.997
rs2297538	G	2757(0.865)	2875(0.869)	0.147	0.701	1.03[0.89–1.87]
	A	429(0.135)	435(0.131)
	G/G	1197(0.751)	1257(0.760)	0.518	0.772
	A/G	363(0.228)	361(0.218)
	A/A	33(0.021)	37(0.022)
	H-W	0.376	0.070
rs28448745	C	2153(0.676)	2271(0.686)	0.735	0.391	1.05[0.94–1.16]
	A	1033(0.324)	1041(0.314)
	C/C	723(0.454)	788(0.476)	1.976	0.372
	A/C	707(0.444)	695(0.420)
	A/A	163(0.102)	173(0.104)
	H-W	0.610	0.284
rs1547354	G	2744(0.861)	2862(0.865)	0.157	0.692	1.03[0.89–1.19]
	A	442(0.139)	448(0.135)
	G/G	1191(0.748)	1238(0.748)	1.637	0.441
	A/G	362(0.227)	386(0.233)
	A/A	40(0.025)	31(0.019)
	H-W	0.050	0.886
rs1292037	T	1802(0.566)	1997(0.603)	9.155	0.002	1.17[1.06–1.29]
	C	1382(0.434)	1315(0.397)
	T/T	522(0.328)	593(0.358)	11.438	0.003
	C/T	758(0.476)	811(0.490)
	C/C	312(0.196)	252(0.152)
	H-W	0.218	0.353
rs13137	A	1801(0.565)	1992(0.601)	8.740	0.003	1.16 [1.05–1.28]
	T	1385(0.435)	1320(0.399)
	A/A	520(0.326)	588(0.355)	11.257	0.004
	A/T	761(0.478)	816(0.493)
	T/T	312(0.196)	252(0.152)
	H-W	0.264	0.258
rs1527423	A	2566(0.805)	2677(0.808)	0.086	0.769	0.98 [0.87–1.11]
	G	620(0.195)	635(0.192)
	A/A	1027(0.645)	1085(0.655)	1.244	0.537
	A/G	512(0.321)	507(0.306)
	G/G	54(0.034)	64(0.039)
	H-W	0.312	0.620

Abbreviations: SNP, single nucleotide polymorphisms; T2DM, type 2 diabetes; NDM, nondiabetic.

Table 3 Comparison of Genotypic and Allelic Distribution of Four SNPs in microRNA-222 Between T2DM Group and NDM Group in Female Subjects

SNPs	Alleles/Genotypes	T2DM n(Freq)	NDM n(Freq)	Chi^2	P value	Odds Ratio[95% CI]
rs2858061	G	962(0.799)	948(0.780)	1.369	0.242	0.89 [0.73–1.08]
	C	242(0.201)	268(0.220)
	G/G	389(0.646)	367(0.604)	2.943	0.230
	C/G	184(0.306)	214(0.352)
	C/C	29(0.048)	27(0.044)
	H-W	0.235	0.550
rs34678647	G	976(0.811)	978(0.804)	0.157	0.692	1.04 [0.85–1.28]
	T	228(0.189)	238(0.196)
	G/G	404(0.671)	400(0.658)	0.279	0.870
	G/T	168(0.279)	178(0.293)
	T/T	30(0.050)	30(0.049)
	H-W	0.026	0.084
rs2858060	C	966(0.801)	950(0.781)	1.428	0.232	1.13[0.93–1.37]
	G	240(0.199)	266(0.219)
	C/C	388(0.643)	369(0.607)	1.737	0.420
	C/G	190(0.315)	212(0.349)
	G/G	25(0.041)	27(0.044)
	H-W	0.775	0.619
rs2745709	C	742(0.615)	736(0.605)	0.254	0.614	1.04 [0.89–1.23]
	T	464(0.385)	480(0.395)
	C/C	232(0.385)	220(0.362)	0.868	0.648
	C/T	278(0.461)	296(0.487)
	T/T	93(0.154)	92(0.151)
	H-W	0.520	0.642

Abbreviations: SNP, single nucleotide polymorphisms; T2DM, type 2 diabetes; NDM, nondiabetic.

Table 4 Different Inheritance Models of rs1292037 and rs13137 in microRNA-21 Between NDM Group and T2DM Group

SNPs	Models	Genotypes	NDM n(Freq)	T2DM n(Freq)	OR (95% CI)	P value	AIC	BIC
rs1292037	Codominant	T/T	593 (0.358)	522 (0.328)	1.00	0.004	4499.6	4530.0
		T/C	811 (0.490)	758 (0.476)	1.06 (0.91–1.24)
		C/C	252 (0.152)	312 (0.196)	1.40 (1.14–1.72)
	Dominant	T/T	593 (0.358)	522 (0.328)	1.00	0.072	4505.5	4529.9
		T/C-C/C	1063 (0.642)	1070 (0.672)	1.14 (0.99–1.32)
	Recessive	T/T-T/C	1404 (0.848)	1280 (0.804)	1.00	0.001	4498.2	4522.5
		C/C	252 (0.152)	312 (0.196)	1.35 (1.13–1.63)
	Overdominant	T/T-C/C	845 (0.510)	834 (0.524)	1.00	0.450	4508.2	4532.5
		T/C	811 (0.490)	758 (0.476)	0.95 (0.83–1.09)
	Log-additive	—	—	—	1.16 (1.05–1.28)	0.003	4499.8	4524.2
rs13137	Codominant	A/A	588 (0.355)	520 (0.326)	1.00	0.004	4501.2	4531.6
		A/T	816 (0.493)	761 (0.478)	1.05 (0.90–1.23)
		T/T	252 (0.152)	312 (0.196)	1.40 (1.14–1.71)
	Dominant	A/A	588 (0.355)	520 (0.326)	1.00	0.087	4507.3	4531.6
		A/T-T/T	1068 (0.645)	1073 (0.674)	1.14 (0.98–1.31)
	Recessive	A/A-A/T	1404 (0.848)	1281 (0.804)	1.00	0.001	4499.7	4524.0
		T/T	252 (0.152)	312 (0.196)	1.35 (1.13–1.62)
	Overdominant	A/A-T/T	840 (0.507)	832 (0.522)	1.00	0.400	4509.5	4533.9
		A/T	816 (0.493)	761 (0.478)	0.94 (0.82–1.08)
	Log-additive	—	—	—	1.16 (1.05–1.28)	0.003	4501.7	4526.0

Abbreviations: T2DM, type 2 diabetes; NDM, nondiabetic; AIC, Akaike information criterion; BIC, Bayesian information criterion.

Table 5 Haplotype-Analysis of rs1292037-rs13137 in the microRNA-21 Between T2DM Group and NDM Group

rs1292037-rs13137	T2DM n(Freq)	NDM n(Freq)	Chi^2	P value	Odds Ratio [95% CI]
C T	1374.98(0.432)	1314.00(0.397)	8.848	0.003	1.16 [1.05–1.28]
T A	1792.98(0.563)	1991.00(0.601)	8.848	0.003	0.86 [0.78–0.95]

Note: All those frequency<0.03 will be ignored in analysis.

Abbreviations: T2DM, type 2 diabetes; NDM, nondiabetic.

Figure 1 The genotype of rs78312845 in microRNA-195 with fasting plasma glucose (FPG) (mmol/L) and glycosylated haemoglobin (HbA1C)(%) in the nondiabetic group (NDM) group. (A) rs78312845 in the microRNA-195G/G genotype was associated with lower FPG than the A/G and A/A genotypes (P=0.012 and P=0.019). (B) rs78312845 in the microRNA-195G/G genotype was associated with lower HbA1C than that in the A/G and A/A genotypes (P=0.008 and P=0.011).

American Diabetes A. 2. Classification and Diagnosis of Diabetes. Diabetes Care. 2017;40(Suppl1):S11–S24. doi:10.2337/dc17-S005.

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