https://orcid.org/0000-0002-7678-5671
Figures & data
Figure 1 Dose-dependent effects of insulin on IL-23 expression in human adipocytes. In vitro differentiated human adipocytes were incubated for 24 hours with saline (control), low-dose (0.1 µM) and high-dose (5 µM) insulin and then PCR analysis of IL-23 mRNA expression was performed. Data are expressed as means ± SD of five independent experiments. *P<0.05; **P< 0.01.
Figure 2 Insulin upregulates the IL-23 protein expression in human adipocytes. In vitro differentiated human adipocytes were incubated for 24 hours with saline (control) and insulin (5 µM). (A) Representative western blot analysis showing IL-23 protein expression. (B) Western blot densitometric quantification of IL-23 protein expression as normalized to β-actin. Control = saline. Values are expressed as mean ±SD from five independent experiments. *P<0.01.
Figure 3 Insulin does not stimulate LL-37 mRNA expression in human adipocytes. In vitro differentiated human adipocytes were incubated for 24 hours with saline (control) and insulin (5 µM) and then PCR analysis of LL-37 mRNA expression was performed. Data are mean ± SD of five independent experiments.
Figure 4 LPS does not stimulate IL-23 mRNA expression in human adipocytes. In vitro differentiated human adipocytes were incubated for 24 hours with saline (control) and LPS (1 ng/mL) and then PCR analysis of IL-23 mRNA expression was performed. Data are expressed as mean ± SD of five independent experiments.
