The Influence of SLC22A3 Genetic Polymorphisms on Susceptibility to Type 2 Diabetes Mellitus in Chinese Population

Figures & data

Table 1 The Demographic and Clinical Characteristics of T2DM Patients and Healthy Controls

Parameter	Healthy Controls (n=220)	T2DM Patients (n=450)	P value
Gender
Male	111	281	0.003*
Female	109	169
Age(years)	52.92±12.83	56.98±13.60	<0.001**
Height(cm)	165.75±8.21	165.48±10.20	0.736
Weight(kg)	63.02±8.89	67.58±11.81	<0.001**
BMI(kg/m^2)	22.88±2.35	25.72±27.52	0.127
HbA1c(%)	5.49±2.68	8.65±2.38	<0.001**
FBG(mmol/L)	5.31±1.37	8.60±3.20	<0.001**
PBG(mmol/L)	6.51±2.94	14.10±5.87	<0.001**
CPO(ng/mL)	2.64±1.43	2.38±1.71	0.091
TC(mmol/L)	5.12±4.25	4.62±1.26	0.024*
TG(mmol/L)	1.78±3.76	2.07±2.12	0.203
LDL(mmol/L)	3.01±4.68	2.81±3.03	0.530
HDL(mmol/L)	1.45±0.45	3.40±47.96	0.545

Notes: Data are given as mean ± SD. Data were analyzed by independent sample T-test. *P<0.05, **P<0.001. The numbers in bold indicated statistically significant values.

Abbreviations: BMI, body mass index; HbA1c, glycosylated haemoglobin; FBG, fasting blood-glucose; PBG, postprandial blood glucose; CPO, C-Peptide; TC, total cholesterol; TG, triglycerides; LDL, low-density lipoprotein cholesterol; HDL, high-density lipoprotein cholesterol; T2DM, type 2 diabetes mellitus.

Figure 1 The experimental design routes of this study.

Table 2 Comparisons of Allelic Frequencies of SLC22A3 rs555754, rs3123636 and rs3088442 Polymorphisms in T2DM Patients and Healthy Controls

Genotype	Healthy Controls (n=220)	T2DM Patients (n=450)	P value
SLC22A3 rs555754
GG	106(48.2%)	183(40.7%)	0.034
GA	95(43.2%)	199(44.2%)
AA	19(8.6%)	68(15.1%)
Alleles
G	307(69.8%)	565(62.8%)	0.012
A	133(30.2%)	335(37.2%)
HWE P	0.940	0.522
SLC22A3 rs3123636
TT	113(51.4%)	276(61.3%)	0.045
TC	91(41.3%)	151(33.6%)
CC	16(7.3%)	23(5.1%)
Alleles
T	343(78.0%)	733(81.4%)	0.014
C	97(22.0%)	167(18.6%)
HWE P	0.923	0.924
SLC22A3 rs3088442
GG	54(24.5%)	137(30.5%)	0.275
GA	119(54.1%)	223(49.7%)
AA	47(21.4%)	89(19.8%)
Alleles
G	227(51.6%)	497(55.3%)	0.195
A	213(48.4%)	401(44.7%)
HWE P	0.469	0.995

Notes: Data were analyzed by chi-square (χ²) test. The numbers in bold indicated statistically significant values.

Abbreviations: T2DM, type 2 diabetes mellitus; HWE, Hardy–Weinberg equilibrium.

Table 3 The Association Between SLC22A3 Genetic Polymorphisms and the Risk of T2DM (Adjusted for Age, Gender and BMI)

SNPs	Comparisons	Test of Association OR (95% CI)	P values
rs555754	AA vs GG (Homozygote comparison)	1.409(1.049–1.892)	0.023
	GA vs GG (Heterozygote comparison)	1.141(0.796–1.636)	0.473
	GA/AA vs GG (Dominant model)	1.335(0.956–1.347)	0.149
	AA vs GA/GG (Recessive model)	1.365(1.032–1.805)	0.029
rs3123636	CC vs TT (Homozygote comparison)	0.645(0.449–0.927)	0.018
	TC vs TT (Heterozygote comparison)	0.727(0.508–1.041)	0.082
	TC/CC vs TT (Dominant model)	0.824(0.694–0.978)	0.027
	CC vs TC/TT (Recessive model)	0.705(0.497–0.999)	0.049
rs3088442	AA vs GG (Homozygote comparison)	0.807(0.621–1.048)	0.107
	GA vs GG (Heterozygote comparison)	0.750(0.500–1.126)	0.165
	GA/AA vs GG (Dominant model)	0.858(0.707–1.040)	0.858
	AA vs GA/GG (Recessive model)	0.910(0.737–1.123)	0.380

Notes: Data were analyzed by binary logistic regression. The numbers in bold indicated statistically significant values.

Abbreviations: OR, odds ratio; CI, confidence interval.

Table 4 Haplotype Analysis of SLC22A3 rs3088442 and rs3123636 with the Risk of T2DM

Haplotypes^a	Case Frequency	Control Frequency	OR (95% CI)	P value
AC	0.212	0.274	0.713(0.548–0.929)	0.011
AT	0.235	0.210	1.155(0.876–1.523)	0.305
GT	0.546	0.510	1.160(0.923–1.459)	0.203

Notes: Data were analyzed by the online software, SHEsis.^{10 a}Only haplotypes with frequency≥0.05 were shown. The numbers in bold indicated statistically significant values.

Abbreviations: T2DM, type 2 diabetes mellitus; OR, odds ratio; CI, confidence interval.

Gu Y, Xu ZJ, Zhou JD, et al. SLC22A3 methylation-mediated gene silencing predicts adverse prognosis in acute myeloid leukemia. Clin Epigenetics. 2022;14(1):162. doi:10.1186/s13148-022-01373-w

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Data Sharing Statement

The data used to support the findings of this study are available from the corresponding author upon request.