Circulating MicroRNA-30a, Beclin1 and Their Association with Different Variables in Females with Metabolically Healthy /Unhealthy Obesity

Figures & data

Table 1 Comparison of Clinical and Metabolic Characteristics of Women with MHO and MUO

Variable	Women with MHO (n=34)	Women with MUO (n=34)	*P value
Age (years)	28.35±7.02	36.47±7.50	< 0.001
Body mass index (kg/m2)	40.82±8.00	43.88±8.06	0.239
Waist circumference (cm)	114.82±14.55	117.97±13.83	1.000
Systolic blood pressure (mmHg)	122.41±18.52	118.18±16.03	0.317
Diastolic blood pressure (mmHg)	81.18±11.82	74.85±10.11	0.0206
Alanine transaminase (U/L)	15.24±5.53	26.50±12.54	< 0.001
Aspartate transaminase (U/L)	21.09±6.53	25.68±8.19	0.013
Fasting plasma glucose (mg/dl)	93.09±4.54	114.35±39.74	0.002
HbA1c % (mmol/mol)	5.23±0.36 (34±2.3)	6.44±1.22 (47±1.3)	< 0.001
TSH (mIU/L)	1.99±1.08	2.73±0.98	0.006
Total cholesterol (mg/dL)	182.65±31.87	201.47±43.73	0.0466
LDL-C (mg/dL)	2.96±0.66	2.96±0.66	0.330
HDL-C (mg/dL)	1.17±0.25	1.09±0.19	0.350
Triglycerides (mg/dL)	2.32±0.49	3.83±1.95	< 0.001
MicroRNA-30a	3.01±1.30	6.84±1.97	< 0.001
Beclin1 (ng/mL)	7.19±1.69	4.34±1.16	< 0.001

Notes: Values are mean ± SD. *P value <0.05 is considered significant.

Abbreviations: HbA1c, glycated hemoglobin; TSH, thyroid stimulating hormone; LDL-C, low density lipoprotein cholesterol; HDL-C, high density lipoprotein cholesterol.

Figure 1 Mean MicroRNA-30a expression level in healthy controls, women with MHO and MUO.

Notes: Women with metabolically healthy obesity (MHO) had significantly higher microRNA-30a (3.01±1.30) than healthy controls (1.03±0.05) (*P<0.001), but significantly lower microRNA-30a compared to women with metabolically unhealthy obesity (MUO) (6.84±1.97) (*P<0.001).

Figure 2 Mean beclin1 level in healthy controls, women with MHO and MUO.

Notes: Women with metabolically healthy obesity (MHO) had significantly lower serum beclin 1 (7.19±1.69 ng/dL) compared to healthy controls (12.61±1.84 ng/mL) (*P<0.001), but significantly higher serum beclin 1 compared to women with metabolically unhealthy obesity (MUO) (4.34±1.16 ng/dL) (*P<0.001).

Table 2 Correlations of Serum beclin1 and microRNA-30a with Different Variables in Women with Obesity

variable	Beclin1		MicroRNA 30-a
	r	*P value	r	*P value
Age (years)	−0.302	0.012	0.499	<0.001
Body mass index (kg/m2)	−0.403	<0.001	0.363	0.002
Waist circumference (cm)	−0.108	0.377	0.253	0.036
Diastolic blood pressure (mmHg)	−0.538	<0.001	0.638	<0.001
Alanine aminotransferase (U/L)	−0.237	0.050	0.408	<0.001
Aspartate aminotransferase (U/L)	−0.208	0.086	0.191	0.116
Fasting plasma glucose (mg/dL)	−0.376	0.001	0.497	<0.001
HbA1c (%)	−0.585	<0.001	0.551	<0.001
TSH (mIU/L)	−0.268	0.026	0.171	0.160
Cholesterol (mg/dL)	−0.178	0.144	0.096	0.431
LDL-C (mg/dL)	−0.111	0.362	0.070	0.565
HDL-C (mg/dL)	−0.003	0.981	−0.163	0.180
Triglycerides (mg/dL)	−0.327	0.006	0.495	<0.001
Beclin 1 (ng/mL)	–	–	−0.654	<0.001
MicroRNA 30-a	−0.654	<0.001	–	–

Note: *P value <0.05 is considered significant.

Abbreviations: HbA1c, glycated hemoglobin; TSH, thyroid-stimulating hormone; LDL-C, low-density lipoprotein cholesterol; HDL-C, high-density lipoprotein cholesterol.

Table 3 Univariate Analysis of Factors Associated with Metabolically Unhealthy Obesity

Variable	*P value	OR	95% C.I.
			Lower	Upper
Age	< 0.001	1.162	1.072	1.260
Alanine transaminase	< 0.001	1.177	1.079	1.284
Thyroid stimulating hormone	0.007	1.981	1.211	3.243
MicroRNA-30a	< 0.001	3.965	2.154	7.298
Beclin1	< 0.001	0.299	0.177	0.506

Note: *P value <0.05 is considered significant.

Table 4 Multivariate Regression Analysis of Factors Associated with Metabolically Unhealthy Obesity

Variable	*P value	OR	95% C.I.
			Lower	Upper
MicroRNA-30a	0.021	6.099	1.317	28.252
Beclin1	0.070	0.138	0.016	1.175

Note: *P value <0.05 is considered significant.

Data Sharing Statement

The data supporting the findings of this study are available from the corresponding author upon reasonable request.