Expression of gluconeogenic enzymes and 11β-hydroxysteroid dehydrogenase type 1 in liver of diabetic mice after acute exercise

Figures & data

Table 1 Basic physical characteristics of lean and diabetic db/db mice

Parameter	Body weight (g)	Liver weight (mg)	Plasma glucose (mg/dL)
Lean mice	21.2±0.4	871±27	164±20
db/db mice	30.8±0.7*	1,714±101*	420±19*

Notes: Body weight represents combined weight of mice from series 1 and 2. Liver weight is representative of mice from series 2 that were euthanized 8 hours after acute exercise. Plasma for the measurement of glucose was obtained in mice prior to exercise in series 1. Values are expressed as mean ± SEM.

* P<0.001 compared to lean mice.

Abbreviation: SEM, standard error of the mean.

Figure 1 Effect of a 30 minute bout of exercise on plasma glucose (A) and insulin (B) in db/db mice.

Notes: Plasma measurements were obtained immediately after exercise. Values are reported as a mean ± standard error of the mean for ten mice in each group. *P<0.05 compared to lean control; ^†P<0.05 compared to basal conditions.

Figure 2 Effect of a 30 minute bout of exercise on plasma NEFA (A) and corticosterone (B) in db/db mice.

Notes: Plasma measurements were obtained immediately after exercise. Values are reported as a mean ± standard error of the mean for ten mice in each group. *P<0.05 compared to lean control; ^†P<0.05 compared to basal conditions.
Abbreviation: NEFA, non-esterified fatty acids.

Figure 3 Plasma levels of corticosterone (A) and adrenocorticotropic hormone (ACTH) (B) measured 8 hours after acute exercise in db/db mice.

Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group. *P<0.05 compared to lean control; ^†P<0.05 compared to lean exercise mice; ^‡P<0.05 compared to db/db mice.

Figure 4 Hepatic protein expression of the glucocorticoid receptor (GR) measured 8 hours after acute exercise in db/db mice.

Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group, and normalized to internal control. *P<0.05 compared to lean control; ^†P<0.05 compared to lean exercise mice.

Figure 5 Hepatic protein expression of phosphoenolpyruvate carboxykinase (PEPCK) measured 8 hours after acute exercise in db/db mice.

Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group, and normalized to internal control. *P<0.05 compared to lean control.

Figure 6 Hepatic protein expression of glucose-6-phosphatase α (G6Pase α) measured 8 hours after acute exercise in db/db mice.

Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group, and normalized to internal control.

Figure 7 Hepatic protein expression of glucose-6-phosphatase β (G6Pase β) measured 8 hours after acute exercise in db/db mice.

Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group, and normalized to internal control.

Figure 8 Hepatic protein expression of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD type 1) measured 8 hours after acute exercise in db/db mice.

Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group, and normalized to internal control. *P<0.05 compared to lean control; ^†P<0.05 compared to lean exercise mice.