Genistein treatment increases bone mass in obese, hyperglycemic mice

Figures & data

Figure 1 The effects of genistein treatment on body mass and serum glucose in ob/ob mice.

Notes: There was no difference between control and genistein-fed mice in body mass (50.9±2.1 g and 46.3±1.2 g, respectively, P=0.06) or serum glucose in control (465.1±22.1 mg/dL and 408.7±37.5 mg/dL, respectively, P=0.22). Data are shown as mean ± 2 SE.
Abbreviation: SE, standard error.

Table 1 The effects of genistein treatment on cross-sectional geometric properties of the tibial midshaft in ob/ob mice

	Control (n=9)	Treated (n=10)	P-value
Tibial length (mm)	16.7±0.11	16.53±0.12	0.41
I max (mm^4×10^−2)	4.78±0.33	4.96±0.25	0.67
I min (mm^4×10^−2)	4.30±0.33	4.47±0.24	0.70
J (mm^4×10^−2)	9.08±0.67	9.47±0.48	0.64
MaxRad (mm)	1.00±0.20	1.22±0.08	0.05
Xbar	0.71±0.08	0.82±0.20	0.21
Ybar	0.70±0.15	0.89±0.19	0.03
PsPm (mm)	3.25±0.06	3.29±0.12	0.78
EsPm (mm)	2.27±0.20	2.20±0.21	0.52
CtAr (mm^2)	0.73±0.03	0.75±0.02	0.53
MAr (mm^2)	0.71±0.02	0.70±0.02	0.76
TtAr (mm^2)	1.44±0.05	1.46±0.04	0.84
CtAr/TtAr (%)	50.5±0.44	51.7±0.34	0.05
MAr/TtAr (%)	49.4±0.43	48.3±0.33	0.05

Notes: Values are expressed as mean ± SE. Bold values represent statistically significant P-values.

Abbreviations: MaxRad, maximum radius; PsPm, periosteal perimeter; EsPm, endosteal perimeter; CtAr, cortical area; TtAr, total area of the cross-section; MAr, medullary area; SE, standard error; I _max, maximum second area of moment; I _min, minimum second area of moment; J, polar moment of inertia; X_bar, X-axis geometric mean; Y_bar, Y-axis geometric mean.

Figure 2 Micrographs of the proximal tibial growth plate of (A) control and (B) genistein-fed ob/ob mice.

Notes: Scale bar is 100 μm. Toluidine blue and aqueous fast green stains were used, 40× magnification.

Figure 3 Growth plate thickness in control and genistein fed mice.

Notes: Growth plate thickness was significantly reduced in genistein-treated mice (42.7±1.98 μm) in comparison with controls (49.3±1.96 μm, P=0.03). There was no difference in the thickness of the calcified cartilage layer between genistein treated (18.6±1.16 μm) and control mice (21.0 ± 1.14 μm, P=0.20). Data are shown as mean ± 2 SE.
Abbreviation: SE, standard error.