Genotyping of HBV and tracking of resistance mutations in treatment-naïve patients with chronic hepatitis B

Figures & data

Table 1 Clinical features of treatment-naïve patients for CHB attending two reference centers for viral hepatitis between 2011 and 2015

Characteristics	Northeast		North
	n=84	Percentage	n=105	Percentage
Age*(min–max, years)	18–72	44.3±12.3	18–72	40.5±12.3
Gender (male/female)	40/39	50.6/49.4	39/66	37.1/62.9
ALT*(IU/L)	7–231	45.65±38.9	12–414	42.63±52.3
RV >41 IU/L	29	38.7	28	28.3
HBeAg (+/−)	10/62	13.9/86.1	07/91	7.1/92.9
HBV DNA log 10 >4	12	15.6	14	13.3
HBV DNA level*	1.51–8.34	3.3±1.47	1.75–8.04	3.12±1.4
HBV genotype
A	75	89.3	62	59
D	2	2.4	22	21
F	6	7.1	21	20
C	1	1.2	0	0
Coinfection
HBV HCV	1	1.2	2	1.9
HBV HDV			4	3.8
Mutation
Vaccine escape (sHBV)	2	2.4	9	8.6
Mutation resistance NA	5	6	0	0

Note:

* Percentage column shown in median and SD and n column shown in min–max.

Abbreviations: ALT, alanine aminotransferase; HBeAg, hepatitis B e antigen; HBV, hepatitis B virus; HCV, hepatitis C virus; HDV, hepatitis D virus; NA, nucleoside/nucleotide analogs; RV, reference value; sHBV, surface antigen gene of hepatitis B virus.

Table 2 Virological and resistance mutation profile of CHB-naïve patients to analog nucleos(t)ides

Patient	Age (years)	Gender	Viral load (log UI/mL)	Genotype	Mutation		HBeAg status	Resistance profile
					rtHBV	sHBV		LAM	ADV	ETV	TDF
H:351	56	M	3.72	A1	S202I		No reagent	S	S	R	S
H:105	62	M	8.6	A1	A194T		No reagent	S	S	S	R
H:117	48	M	2.21	A1	M204I	Q129H	Reagent	R	S	I	S
H:123	27	M	6.11	F2	A181S		Reagent	R	R	S	S
H:226	30	M	8.3	A2	L180M + M204V	I195M	Reagent	R	S	R	S

Abbreviations: ADV, adefovir dipivoxil; CHB, chronic hepatitis B; ETV, entecavir; HBeAg, hepatitis B e antigen; HBV, hepatitis B virus; I, intermediate/reduced susceptibility; LAM, lamivudine; R, resistant; S, susceptible; TDF, tenofovir.

Table 3 Comparing the epidemiological characteristics of patients with resistance mutations and without resistance mutations in the HBV RT region

Characteristics	Group with mutation (n=5)	Group without mutation (n=79)
Genotype
Type A	4	71
Type non-A	1	8
Gender
Male	4	36
Female	1	38
HbeAg
Positive	3	7
Negative	2	60
Age
≥40 years	3	44
≤40 years	2	21
HBV DNA
≥ log 4	2	10
≤ log 4	3	62
ALT
≤ RV =41 IU/mL	2	44
≥ RV =41 IU/mL	3	26

Note: There was no significant association at P<0.05.

Abbreviations: HBeAg, hepatitis B e antigen; HBV, hepatitis B virus; ALT, alanine aminotransferase; RV, reference value; IU, international unit.