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Infection and Drug Resistance Volume 12, 2019 - Issue
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Original Research

Molecular characterization of the pilS2 gene and its association with the frequency of Pseudomonas aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island

Aghil Bahramian1 Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Saeed Khoshnood3 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected];4 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected]Correspondence[email protected]
,
Aref Shariati5 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
,
Farahnoosh Doustdar2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Alireza Salimi Chirani2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
&
Mohsen Heidary5 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;6 Student Research Committee, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
Pages 221-227 | Published online: 11 Jan 2019

Figures & data

Table 1 Primer sequence used in the genetic characterization of Pseudomonas aeruginosa clinical strains

Table 2 Antibiotic resistance patterns in Pseudomonas aeruginosa isolates

Figure 1 Detection of pils2 gene (428 bp) in Pseudomonas aeruginosa isolates.

Notes: 1–3 and 5 positive samples, 4 and 6 negative samples; molecular pattern: 100 bp.

Abbreviations: N, negative control; P, positive control.

Figure 1 Detection of pils2 gene (428 bp) in Pseudomonas aeruginosa isolates.Notes: 1–3 and 5 positive samples, 4 and 6 negative samples; molecular pattern: 100 bp.Abbreviations: N, negative control; P, positive control.

Figure 2 Detection of CP44 gene (272 bp) in Pseudomonas aeruginosa isolates.

Note: Molecular pattern: 100 bp.

Abbreviations: N, negative control; P, positive control.

Figure 2 Detection of CP44 gene (272 bp) in Pseudomonas aeruginosa isolates.Note: Molecular pattern: 100 bp.Abbreviations: N, negative control; P, positive control.

Figure 3 Detection of CP10 gene (255 bp) in Pseudomonas aeruginosa isolates.

Note: Molecular pattern: 100 bp.

Abbreviations: N, negative control; P, positive control.

Figure 3 Detection of CP10 gene (255 bp) in Pseudomonas aeruginosa isolates.Note: Molecular pattern: 100 bp.Abbreviations: N, negative control; P, positive control.

Table 3 Frequency of the specific genes CP10, CP44, and CP97 of pKLC102

Table 4 The association of pilS2 with pKLC102, chromosomal PAPI-1, and plasmid PAPI-1

Table 5 The association of pKLC102 with chromosomal PAPI-1 and plasmid PAPI-1

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