Figures & data
Table 1 Resistance features of the ESBL-producing K. pneumoniae isolates, transconjugants, and recipients
Figure 1 Differential gene expression of multidrug efflux pumps and global regulators among the 30 EPKP isolates.
Notes: Fold change in the expression of genes encoding (A) the multidrug efflux pump AcrB; (B) the multidrug efflux pump OqxB; (C) the multidrug efflux pump KpgB; (D) the global regulator RamA; and (E) the global regulator RarA. Fold changes in gene expression were determined after normalizing to that of the 16S rRNA gene (rrsE) in each strain and then comparing the expression of each gene with corresponding genes in the tigecycline-susceptible Klebsiella pneumoniae strain ATCC 13883.
Abbreviations: EPKP, ESBL-producing K. pneumoniae; ESBL, extended-spectrum β-lactamase.
Figure 2 S1 endonuclease pulsed-field gel electrophoresis analysis of plasmids from the 30 VAP-inducing EPKP isolates in China.
Notes: (A) “M” indicates the molecular weight marker; Lanes 1–11 show the plasmid profiles of isolates EPKP1–EPKP11, respectively; (B) Lanes 12–22 show the plasmid profiles of isolates EPKP12–EPKP22, respectively; and (C) Lanes 23–30 show the plasmid profiles of isolates EPKP23–EPKP30, respectively.
Abbreviations: VAP, ventilator-associated pneumonia; EPKP, ESBL-producing Klebsiella pneumoniae; ESBL, extended-spectrum β-lactamase.
Table 2 Antibiotic resistance genes and plasmid replicon types in transferable blaCTX-15-producing Klebsiella pneumoniae donors and their transconjugants
Figure 3 Genetic relatedness, plasmid size, and replicon type of the 30 VAP-inducing EPKP isolates in China.
Note: Dendrogram of patterns generated by PFGE.
Abbreviations: MLST, multilocus sequence typing; ST, sequence type; –, not detected; VAP, ventilator-associated pneumonia; EPKP, ESBL-producing K. pneumoniae; ESBL, extended-spectrum β-lactamase; PFGE, pulsed-field gel electrophoresis.
