Figures & data
Table 1 Antibiotic susceptibility of the E. cloacae PIMB10EC27
Table 2 Distribution of coding genes conferring to antibiotic resistance in PIMB10EC27
Figure 1 Structure of plasmid pEC27-1 and pEC27-2. pEC27-1 is an IncX3 plasmid that possesses highly syntenic plasmid backbone compare to IncHI1 plasmid pEC27-2. The outer circle shows ORFs on forward and reverse strands. Resistance genes, transposase genes and resolvase genes are depicted by red, black, blue arrows, respectively. The two inner circles show the GC content (purple circle) and GC skew (blue indicates positive values, red indicates negative values) information.
![Figure 1 Structure of plasmid pEC27-1 and pEC27-2. pEC27-1 is an IncX3 plasmid that possesses highly syntenic plasmid backbone compare to IncHI1 plasmid pEC27-2. The outer circle shows ORFs on forward and reverse strands. Resistance genes, transposase genes and resolvase genes are depicted by red, black, blue arrows, respectively. The two inner circles show the GC content (purple circle) and GC skew (blue indicates positive values, red indicates negative values) information.](/cms/asset/cdf8d4da-78cc-42e9-8f0a-c5686f96b1b8/didr_a_194495_f0001_c.jpg)
Figure 2 Genetic context of blaNDM-1 on IncX3 plasmids pKPN5047, pNDM-HF727, pNDM-HN380, RJA274 plasmid NDM-1, and pEC27-1. Gray shading denotes shared regions of homology: light gray indicates a forward match and dark gray indicates a reverse match. Notably, ygbJ is split into two parts, and the IS26-ΔygbJ region is inverted in pEC27-1 relative to the other plasmids. The mpr gene in pEC27-1 is interrupted by ISKpn19 and Tn6901 insertions. Open reading frames are portrayed by arrows and are colored. Resistance genes are indicated in red arrows, including blaNDM-1, blaSHV-12, bleMBL, fgh, glo, and adh.
![Figure 2 Genetic context of blaNDM-1 on IncX3 plasmids pKPN5047, pNDM-HF727, pNDM-HN380, RJA274 plasmid NDM-1, and pEC27-1. Gray shading denotes shared regions of homology: light gray indicates a forward match and dark gray indicates a reverse match. Notably, ygbJ is split into two parts, and the IS26-ΔygbJ region is inverted in pEC27-1 relative to the other plasmids. The mpr gene in pEC27-1 is interrupted by ISKpn19 and Tn6901 insertions. Open reading frames are portrayed by arrows and are colored. Resistance genes are indicated in red arrows, including blaNDM-1, blaSHV-12, bleMBL, fgh, glo, and adh.](/cms/asset/71f786be-3fec-4b94-bddc-c173bb03e023/didr_a_194495_f0002_c.jpg)
Data availability
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.