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Infection and Drug Resistance Volume 12, 2019 - Issue
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Original Research

Loop-mediated isothermal amplification for detection of Legionella pneumophila in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran

Mojtaba Moosavian1 Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;2 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected]
,
Sakineh Seyed-Mohammadi2 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected];3 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected]
,
Morteza Saki2 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected];3 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected]
,
Fatemeh Shahi2 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected];3 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected]Correspondence[email protected]
,
Mahtab Khoshkholgh Sima2 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, [email protected]
,
Davoud Afshar4 Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
&
Sara Barati5 Department of Pathobiology, School of Veterinary, University of Shahid Chamran, Ahvaz, Iran
 show all
Pages 529-534 | Published online: 01 Mar 2019

Figures & data

Table 1 Primers used in LAMP and PCR for detection of Legionella pneumophila

Figure 1 Electrophoresis of loop-mediated isothermal amplification products.

Notes: Lane 1, DNA ladder; lanes 2 and 3, negative controls, distilled water; lane 4, negative sample; lane 5, positive control – Legionella pneumophila (ATCC 33152); lanes 6–8, positive samples; lanes 9–12, negative samples.

Figure 1 Electrophoresis of loop-mediated isothermal amplification products.Notes: Lane 1, DNA ladder; lanes 2 and 3, negative controls, distilled water; lane 4, negative sample; lane 5, positive control – Legionella pneumophila (ATCC 33152); lanes 6–8, positive samples; lanes 9–12, negative samples.

Figure 2 Loop-mediated isothermal amplification for detection of Legionella pneumophila in sputum samples with SYBR green added.

Notes: Lane 1, negative control – distilled water; lanes 2 and 3, negative samples; lane 4, positive control – L. pneumophila (ATCC 33152); lanes 5 and 6, positive samples.

Figure 2 Loop-mediated isothermal amplification for detection of Legionella pneumophila in sputum samples with SYBR green added.Notes: Lane 1, negative control – distilled water; lanes 2 and 3, negative samples; lane 4, positive control – L. pneumophila (ATCC 33152); lanes 5 and 6, positive samples.

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