Molecular characterization, serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the Klebsiella pneumoniae strains isolated from different types of hospital-acquired infections

Figures & data

Table 1 Prevalence of K. pneumoniae strains isolated from different types of hospital-acquired infections

Type of samples	No. samples collected	Prevalence of K. pneumoniae (%)
Urine	80	51 (63.75)
Wound	45	15 (33.33)
Blood	64	24 (37.50)
Sputum	71	25 (35.21)
Total	260	115 (44.22)

Abbreviations: K. pneumonia, Kelebsiella pneumonia; NO, number.

Table 2 Antibiotic resistance pattern of K. pneumoniae strains isolated from different types of hospital-acquired infections

Type of samples (No. K. pneumonia strains)	Antibiotic resistance pattern (%)
	Amp*	Amc	Pit	Cfz	Cfr	Az	Imp	Amk	T30	G10	Tob	Cip	Nal	Cot	C30
Urine (51)	51 (100)	51 (100)	47 (92.15)	47 (92.15)	51 (100)	35 (68.62)	27 (52.94)	27 (52.94)	24 (47.05)	39 (76.47)	39 (76.47)	43 (84.31)	47 (92.15)	43 (84.3)	27 (52.94)
Wound (15)	15 (100)	15 (100)	11 (73.33)	15 (100)	15 (100)	7 (46.66)	8 (53.33)	4 (26.66)	4 (26.66)	15 (100)	15 (100)	15 (100)	11 (73.33)	7 (46.66)	3 (20)
Blood (24)	24 (100)	24 (100)	24 (100)	24 (100)	24 (100)	14 (58.33)	24 (100)	24 (100)	19 (79.16)	19 (79.16)	24 (100)	24 (100)	19 (79.16)	19 (79.16)	10 (41.66)
Sputum (25)	25 (100)	20 (80)	15 (60)	25 (100)	25 (100)	25 (100)	10 (40)	10 (40)	15 (60)	10 (40)	15 (60)	10 (40)	15 (60)	20 (80)	10 (40)
Total (115)	115 (100)	110 (95.65)	97 (84.34)	111 (96.52)	115 (100)	86 (74.78)	69 (60)	65 (56.52)	62 (53.91)	83 (72.17)	93 (80.86)	92 (80)	92 (80)	89 (77.39)	50 (43.47)

Abbreviations: Amp, ampicillin (10 μg); Amc, amoxicillin/clavulanic acid (20/10 μg); Pit, piperacillin/tazobactam (110/10 μg); Cfz, ceftazidime (30 μg); Cfr, cefuroxime (30 μg); Az, aztreonam (30 μg); Imp, imipenem (10 μg); Amk, amikacin (30 μg); T30, tetracycline (30 μg); G10, gentamicin (10 μg); Tob, tobramycin (10 μg); Cip, ciprofloxacin (5 μg); Nal, nalidixic acid (30 μg); Cot, co-trimoxazole (30 μg); C30, chloramphenicol (30 μg); K. penumonia, Klebsiella pneumonia.

Table 3 Distribution of virulence genes amongst the K. pneumoniae strains isolated from different types of hospital-acquired infections

Type of samples (No. K. pneumoniae strains)	Distribution of virulence genes (%)
	rmpA	fimH-1	mrkD	traT	entB	jrp1	iuCc
Urine (51)	12 (23.52)	43 (84.31)	47 (92.15)	47 (92.15)	47 (92.15)	8 (15.68)	12 (23.52)
Wound (15)	4 (26.66)	15 (100)	11 (73.33)	15 (100)	7 (46.66)	4 (26.66)	7 46.66()
Blood (24)	–	24 (100)	24 (100)	24 (100)	24 (100)	14 (58.33)	10 (29.41)
Sputum (25)	–	25 (100)	15 (60)	20 (80)	15 (60)	10 (40)	10 (40)
Total (115)	16 (13.91)	107 (93.04)	97 (84.34)	106 (92.17)	93 (80.86)	36 (31.30)	39 (33.91)

Abbreviation: K. pneumonia, Klebsiella pnuemonia.

Table 4 Distribution of antibiotic resistance genes amongst the K. pneumoniae strains isolated from different types of hospital-acquired infections

Type of samples (No. K. pneumoniae strains)	Distribution of antibiotic resistance genes (%)
	ompK35	ompK36	mdtK	tolC	acrAB
Urine (51)	43 (84.31)	47 (92.15)	8 (15.68)	43 (84.31)	51 (100)
Wound (15)	15 (100)	15 (100)	11 (73.33)	11 (73.33)	11 (73.33)
Blood (24)	19 (79.16)	15 (62.50)	10 (41.66)	24 (100)	24 (100)
Sputum (25)	10 (40)	15 (60)	5 (20)	20 (80)	25 (100)
Total (115)	87 (75.65)	91 (79.13)	34 (29.54)	98 (85.21)	111 (96.52)

Abbreviation: K. pneumonia, Klebsiella pnuemonia.

Table 5 Distribution of capsule serotypes amongst the K. pneumoniae strains isolated from different types of hospital-acquired infections

Type of samples (No. K. pneumoniae strains)	Distribution of antibiotic resistance genes (%)
	K1	K2	Non K1/K2
Urine (51)	12 (23.52)	4 (7.84)	35 (68.62)
Wound (15)	–	4 (26.66)	11 (73.33)
Blood (24)	10 (41.66)	–	14 (58.33)
Sputum (25)	10 (40)	–	15 (60)
Total (115)	32 (27.82)	8 (6.95)	75 (65.21)

Abbreviation: K. pneumonia, Klebsiella pnuemonia.