Genetic characterization of two vancomycin-resistant Staphylococcus aureus isolates in Kerman, Iran

Figures & data

Table 1 The primer sequences were used in this study

Gene target	Primer sequence(5ʹ-3ʹ)	Annealing temperature (°C)	Product (bp)	Ref
nuc	F-GCGATTGATGGTGATACGGTT	60	279	^Citation11,Citation12
	R-AGCCAAGCCTTGACGAACTAAAGC
mecA	F-TCCAGATTACAACTTCACCAGG	56	162	^Citation11,Citation12
	R-CCACTTCATATCTTGTAACG
vanA	F-CATGAATAGAATAAAAGTTGCAATA	50	1030	^Citation12
	R-CCCCTTTAACGCTAATACGATCAA
erm A	F-TATCTTATCGTTGAGAAGGGATT	56.5	139	^Citation11,Citation12
	R-CTACACTTGGCTTAGGATGAAA
erm B	F-CTATCTGATTGTTGAAGAAGGATT	56.5	142	^Citation11,Citation12
	R-GTTTACTCTTGGTTTAGGATGAAA
erm C	F-AATCGTCAATTCCTGCATGT	55.5	297	^Citation11,Citation12
	R-TAATCGTGGAATACGGGTTTG
msrA	F-GCAAATGGTGTAGGTAAGACAACT	56.5	402	^Citation11,Citation12
	R-ATCATCATGTGATGTAAACAAAAT
aac(6ʹ)-Ie-aph(2ʹ’)-I	F-CAGGAATTTATCGAAAATGGTAGAAAAG	60	369	^Citation14
	R-CACAATCGACTAAAGAGTACCAATC
aph(2ʹ’)-Ib	F-CTTGGACGCTGAGATATATGAGCAC	60	867	^Citation14
	R-GTTTGTAGCAATTCAGAAACACCCTT
aph(2ʹ’)-Ic	F-CCACAATGATAATGACTCAGTTCCC	60	444	^Citation14
	R-CCACAGCTTCCGATAGCAAGAG
aph(2ʹ’)-Id	F-GTGGTTTTTACAGGAATGCCATC	60	641	^Citation14
	R-CCCTCTTCATACCAATCCATATAACC
aph(3ʹ)-IIIa	F-GGCTAAAATGAGAATATCACCGG	60	523	^Citation14
	R-CTTTAAAAAATCATACAGCTCGCG
ant(4ʹ)-Ia	F-CAAACTGCTAAATCGGTAGAAGCC	60	294	^Citation14
	R-GGAAAGTTGACCAGACATTACGAACT
chp	F-GAAAAAGAAATTAGCAACAACAG	48	410	^Citation15
	R-CATAAGATGATTTAGACTCTCC
sak	F-AAGGCGATGACGCGAGTTAT	50	223	^Citation15
	R-GCGCTTGGATCTAATTCAAC
sea	F-AGATCATTCGTGGTATAACG	50	408	^Citation15
	R-TTAACCGAAGGTTCTGTAGA
sep	F-AATCATAACCAACCGAATCA	50	500	^Citation15
	R-TCATAATGGAAGTGCTATAA
scn	F-AGCACAAGCTTGCCAACATCG	49	258	^Citation15
	R-TTAATATTTACTTTTTAGTGC
icaA	F-TCTCTTGCAGGAGCAATCAA	60	188	^Citation15
	R-TCAGGCACTAACATCCAGCA
icaB	F-ATGGCTTAAAGCACACGACGC	61	526	^Citation15
	R-TATCGGCATCTGGTGTGACAG
icaC	R-CTCTCTTAACATCATTCCGACGCC	63	1013	^Citation15
	F-ATCATCGTGACACACTTACTAACG
icaD	F-GAACCGCTTGCCATGTGTTG	61	483	^Citation15
	R-GCTTGACCATGTTGCGTAACC
pvl	F-TCATTAGGTAAAATGTCTGGACATGATCCA	55	433	^Citation11,Citation12
	R-GCATCAASTGTATTGGATAGCAAAAGC
agr	Pan-ATG CAC ATG GTG CAC ATG C	50	441 575 323 659	^Citation16
	I-GTCACAAGTACTATAAGCTGCGAT
	II-TATTACTAATTGAAA AGT GGC CATAGC
	III-GTA ATG TAATAGCTTGTATAATAATACCCAG
	IV-CGATAATGCCGTAATACCCG

Table 2 Primers used for amplification and sequencing of van operon

Name of primers	Primer sequence(5ʹ-3ʹ)	Annealing temperature (°C)	PCR product (bp)	Ref
Tn1	F-CCATTTTCGCTCCTCTAACG	50.5	1025	This study
	R-CGAATCTGGAAAGCGAAAAG
Tn2	F-CTTTTCGCTTTCCAGATTCG	51	802
	R-AGAATGGTGTGGGAAGCAAG
TnRe	F-GATGGATGCTGCGAGGTAAT	51.5	849
	R-ATCCATTCCGATCTCGTTCA
Tn,Re,VanR	F-CCCGTTTGGAAAAAGTGAAG	50	1074
	R-TCGGCAATTTCATGTTCATC
VanRS	F-GTATTCCGCTAATGGGCAAT	50	1027
	R-GATCCAATCCCCAAGTTTCC
VanS	F-TTCAATGATCCGAGGGAAAC	52	1009
	R-CGCTGGAAGCTCTACCCTAA
VanS,IS1251	F-ACGCGGAAAGCAATGATAAC	52	1297
	R-TATTGTGCGTTGGGGTACAA
IS1251,VanH	F-GCTGACAAGCTTTGCATTTG	52	1203
	R-CGCTATTTTGCAGCAACTCA
VanHA	F-GCTTATAGTCGCAGCCGAAG	52.5	1273
	R-TTTTGCCGTTTCCTGTATCC
VanAX	F-TTATAACCGTTCCCGCAGAC	52.5	847
	R-TGGGGTATGGTTCGTCTCTT
VanX,IS1216E	F-CATGGAAAACAGTGGGTTTG	51.5	1200
	R-ACGGCACAATCTCGTTTGA
VanY	F-TTGTCAACAGGCAGTTCAGC	51	1143
	R-TTTCATTCCGCCATCCTTAC
VanYZ	F-CACCATTCATGTGCCGACTA	52	862
	R-TTTTCCCCTCACTTCACACC
Rev	F-CCCTTCACGTTGTCTCATCC		Pan

Figure 1 Agarose gel electrophoresis of vanA gene detected in two VRSA strains.

Figure 2 A population snapshot of the entire S. aureus with 5 or more nearest match of seven allelic profiles with ST239 in http://bigsdb.pasteur.fr MLST database showing clonal complexes (CCs) viewed using eBURST.

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