Escherichia coli belonging to ST131 rarely transfers bla_ctx-m-15 to fecal Escherichia coli

Figures & data

Table 1 Conjugation frequencies and genomic characterization of donors (phylogroup, MLST type, O-antigen, fimH-type, and Inc-group) and recipients (phylogroup, MLST type, O-antigen, Inc-groups and restriction/modification systems)

blaCTX-M-15 Donor				Hvi15 (A, ST10, O28, fimH24, IncI1)			Hvi09 (B2, ST131, O25, fimH30, IncFIA, IncFII)			Hvi132 (B2, ST131, O25, fimH35, IncFIA)
Recipient	Molecular typing^a	Plasmid Inc-groups	R/M-systems	TK/recipient^b	TK/donor	n	TK/recipient	TK/donor	n	TK/recipient	TK/donor	n
KTE6	B2, ST131, O25	IncFIB, Col156, IncFII, IncX1	2 Type I	<1 x 10^−8	<4 x 10^−10	4	ND	ND		<1 x 10^−8	<8 x 10^−9	4
KTE49	B2, ST131, O25	IncFII, IncFIA, IncFIB	3 Type I	4 x 10^−9	2 x 10^−9	20	ND	ND		ND	ND
Hvi138	B2, ST131, O25	IncFIB, IncFII, Col156	2 type I	1 x 10^−7	4 x 10^−8	11	ND	ND		ND	ND
KTE67	B2, ST73, O2	-	1 Type I	<1 x 10^−7	<4 x 10^−10	4	ND	ND		ND	ND
KTE72	B2, ST141, O2	Col156, Inc Y	1 Type I	3 x 10^−8	2 x 10^−7	2	<5 x 10^−10	<2 x 10^−9	4	<5 x 10^−10	<7 x 10^−8	8
KTE113	B2, ST141, O2	-	1 Type I	4 x 10^−8	4 x 10^−9	3	<1 x 10^−9	<9 x 10^−10	4	<4 x 10^−10	<1 x 10^−8	4
KTE168	B2, ST141, O2	-	2 Type I	5 x 10^−8	1 x 10^−7	7	<4 x 10^−10	<9 x 10^−9	4	<1 x 10^−9	<3 x 10^−9	4
KTE64	NT, ST399, O8	IncFIB, p0111	1 Type II 1 Type IV	<1 x 10^−9	<1 x 10^−9	4	ND	ND		ND	ND
KTE114	D, ST3568, O36	IncX4, p0111, IncI1	2 type I	ND	ND		<8 x 10^−10	<1 x 10^−9	4	<1 x 10^−9	<2 x 10^−9	4
KTE144	D, ST2797, O120	-	1 type I 1 Dnd-system	2 x 10^−8	1 x 10^−8	21	<7 x 10^−10	<7 x 10^−10	4	<1 x 10^−9	<8 x 10^−10	12
KTE165	B2, ST1236, O62	-	1 Type IV 1 Type I	<2 x 10^−7	<6 x 10^−10	4	ND	ND		ND	ND
KTE171	A, ST227, O52	-	-	7 x 10^−7	9 x 10^−10	2	<1 x 10^−9	<6 x 10^−10	4	ND	ND
AL01319	-	-	-	2 x 10^−7	4 x 10^−9	4	ND	ND		<7 x 10^−8	<7 x 10^−10	4

Notes: ^aPhylogroup, MLST type and O-antigen. ^bMedian frequencies were calculated from CFU/mL: (transconjugants ÷ background mutation)/recipient and (÷ background mutation)/donor. Background mutation was zero for all experiments. If no transconjugants were detected, the transfer frequency was listed as <1/recipient and (transconjugants ÷ background mutation)/donor.

Abbreviations: ND, not determined; n, number of CFU counts behind listed median frequencies.

Figure 1 (A) Illustration of the observed difference in conjugation frequencies of recipients belonging to O25 compared to other O-types. (B) Difference in conjugation frequency for one recipient depending on ampicillin selective pressure during conjugation. Statistics: Mann–Whitney test with application of Bonferroni correction to adjust for multiple testing (α=0.017). Frequencies below the detection limit were not plotted. Horizontal lines represent medians.

Table S1 Genomic data on donors and recipients including sequencing method, mean coverage, number of contigs/scaffolds, genome size, accession number

Isolate ID	Seq. method	Genome cov.	Number of scaffolds	Genome size	Database	Accession number
KTE6	PE+MP	143	4	5,213,369	GenBank	NZ_ANVH00000000.1
KTE49	PE+MP	254	6	5,263,728	GenBank	NZ_ANUC00000000.1
KTE64	PE+MP	135	15	5,266,965	GenBank	NZ_ASUV00000000.1
KTE67	PE+MP	272	5	5,029,494	GenBank	NZ_ANUM00000000.1
KTE72	PE+MP	120	15	5,219,548	GenBank	NZ_ANUN00000000.1
KTE113	PE	169	87	5,026,156	GenBank	NZ_ANXQ00000000.1
KTE114	PE+MP	142	12	4,754,090	GenBank	NZ_ASTS00000000.1
KTE144	PE+MP	127	8	4,890,532	GenBank	NZ_ANWL00000000.1
KTE165	PE+MP	139	3	4,757,839	GenBank	NZ_ANWQ00000000.1
KTE168	PE	173	96	5,099,397	GenBank	NZ_ANYM00000000.1
KTE171	PE+MP	138	11	4,859,600	GenBank	NZ_ANVG00000000.1
Hvi138^#	PE+MP	345	17	5,298,611	ENA	PRJEB32936
Hvi132^#	PE+MP	175	28	5,410,850	ENA	PRJEB32936
Hvi09^#	PE+MP	104	17	5,425,536	ENA	PRJEB32936
Hvi15^#	PE	52	128*	4,724,698	ENA	PRJEB32936

Notes: *Number of contigs, ^#Sequenced for this study.

Abbreviations: MP, mate-pair; PE, paired-end.

Data availability

The genomic data of the recipients and donors can be found in GenBank or ENA as described in Table S1.