Figures & data
Figure 1 Total IgG antibodies in the collected sera of BALB/c mice immunized with PBS, pVitro2neo-mcs, CpG-ODN, pVitro-SAG1, pVitro-GRA7, pVitro-SAG1GRA7 and pVitro-SAG1GRA7 with CpG-ODN on days 0, 20, 41 and 62 using ELISA. Results are expressed as means of OD 490± SD and statistically significant differences (P<0.05) are indicated by an asterisk (*), compared to control groups.
Abbreviations: PBS, phosphate buffer saline; ELISA, enzyme-linked immunosorbent assay; OD, optical density; SD, standard deviation.
Figure 2 Levels of IgG2a subtypes in collected sera of BALB/c mice immunized with PBS, pVitro2neo-mcs, CpG-ODN, pVitro-SAG1, pVitro-GRA7, pVitro-SAG1-GRA7 and pVitro-SAG1-GRA7 with CpG-ODN on days 0, 20, 41 and 62 using ELISA. Results are expressed as means of OD 490± SD and statistically significant differences (P<0.05) are indicated by an asterisk (*), compared to control groups.
Abbreviations: ELISA, enzyme-linked immunosorbent assay; OD, optical density; SD, standard deviation.
Figure 3 The IgG2a/IgG1 ratio in mice sera on Day 62 analyzed using ELISA. Statistically significant differences (P<0.05) are indicated by an asterisk (*), compared to control groups.
Abbreviation: ELISA, enzyme-linked immunosorbent assay.
Table 1 Stimulation index four weeks post final immunization assessed using ELISA in sera of immunized mice
Table 2 Cytokine production in vaccinated mice splenocyte cultures four weeks post final immunization using ELISA (pg/mL)
Figure 4 Survival curves in control and experimental groups after receiving 1×103 T. gondii RH strain tachyzoites four weeks after the last immunization. Each group included nine mice. Survival was monitored daily for 18 days after challenges.
