Efficacy Of Line Probe Assay In Detection Of Drug-Resistant Pulmonary Tuberculosis In Comparison With GeneXpert And Phenotypic Methods In Iran And Genetic Analysis Of Isolates By MIRU-VNTR

Figures & data

Table 1 Comparison Of MTB Detection From Clinical Sputum Specimens By Using Culture, LPA, And GeneXpert Method

Case	Culture	LPA	GeneXpert
1	+	+	+
2	+	+	+
3	+	+	+
4	+	+	+
5	+	+	+
6	+	+	+
7	+	+	+
8	+	+	+
9	+	+	+
10	+	+	+
11	+	+	+
12	+	−	+
13	+	+	+
14	−	+	+
15	+	+	+
16	+	+	+
17	+	+	+
18	+	+	+
19	+	+	+
20	−	+	+
21	+	+	+
22	+	+	+
23	+	+	+
24	+	+	+
25	−	+	+
26	+	+	+
27	+	+	+
28	+	+	+
29	–	+	+
30	+	+	+
31	+	+	+
32	+	+	+
33	+	+	+
34	+	−	+
35	+	+	+
36	+	+	+
37	+	+	+
38	−	+	+
39	+	+	+
40	−	+	+
41	+	+	+
42	+	+	+
43	+	+	+
44	+	+	+
45	+	+	+
46	+	+	+
47	+	+	+
48	+	+	+
49	+	+	+
50	+	+	+
51	+	+	+
52	+	+	+
53	+	+	+
54	+	+	+
55	+	+	+
56	+	+	+
57	+	+	+
58	−	−	+
59	+	+	+
60	+	+	+
61	+	+	+
62	+	+	+
63	+	+	+
Total	56 (6.08)*	60 (6.5)*	63 (6.8)*

Note: *The number and % of positive results by each method.

Table 2 The Mutation Of rpoB Gene Among RIF-Resistant Strains And Their MICs

Isolates	Changed Codon	Mutation	MICs (µg/mL)
1	526–529	H526Y	8
2	526–529	H526Y	4
3	530–533	S531L	16
4	505–509	F505L or T508A or S509T*	32

Notes: Bold: MDR isolate. *Possible mutations for 505–509 region.

Table 3 The Mutations Of KatG And inhA Among INH-Resistant Strains And Their MICs

Isolate	Mutation	MICs (µg/mL)
1	KatG (S315T1)+InhA (A–16G)	8
2	KatG (S315T1)+InhA (A–16G)	16
3	KatG (S315T1)+InhA (A–16G)	4
4	InhA (A–16G)	4
5	InhA (A–16G)	2
6	InhA (A–16G)	4
7	InhA (A–16G)	0.125
8	InhA (A–16G)	1
9	InhA (A–16G)	4
10	InhA (A–16G)	1
11	InhA (A–16G)	0.5
12	InhA (A–16G)	2
13	InhA (C–15T)	0.125
14	InhA (C–15T)	0.125

Note: Bold: MDR isolate.

Figure 1 Laboratory performance for each pulmonary sample.

Figure 2 Banding patterns obtained by GenoType MTBDRplus VER 2.0 test. CC; conjugate control, AC; amplification control, TUB; M. tuberculosis complex-specific control, rpoB, katG and inhA; locus control zones specific for each gene. (Sample 8 is positive control of MTB (H37Rv), sample 2 have katG WT (wild type) and katG MUT1 bands which show S315T1 mutation in codon 315 of katG gene; Samples 1, 2, 5, 7, 10, 11, and 12 have inh WT1, 2 and inhA MUT1 bands which show A–16G mutation in inhA gene. Sample 2 has both KatG (S315T1) and inhA (A–16G) mutations simultaneously.

Figure 3 Genetic relatedness of 53 M. tuberculosis isolates by MIRU-VNTR genotyping (0.17 cut-off). Right hand: the allele number of 15 loci in MIRU-VNTR for each isolate. Isolate 1 is M. tuberculosis H37Rv.