Characterization Of bla_NDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China

Figures & data

Table 1 Clinical Characteristics Of bla_NDM-5-Positive E. Coli Strains

Isolate	Sex	Patient Age	Ward	Isolation Time	Specimen	Antimicrobial Treatment	Outcome	MLST	Inc	Size (~kb)
CREC-1	Female	76	General surgery	2017/7/21	Pus	ZOX	Recovered	ST167	X3	50
CREC-2	Female	51	Urology	2017/7/26	Blood	IPM	Death	ST167	X3	50
CREC-4	Female	50	Hepatology	2017/8/4	Pus	CFP/SUL+ATM	Recovered	ST10	X3	50
CREC-6	Female	33	Colorectal surgery	2017/8/10	Pus	CAZ	Recovered	ST540	X3	50
CREC-8	Male	57	Urology	2017/9/1	Urine	CFP/TZP	Recovered	ST448	X3	50
CREC-9	Female	92	Nephrology	2017/9/1	Urine	CFP/SUL	Recovered	ST167	X3	50
CREC-10	Female	53	Nephrology	2017/9/11	Sputum	TZP	Poor	ST410	X3	50
CREC-11	Male	94	Nephrology	2017/9/18	Sputum	MXF+TZP	Death	ST410	X3	50
CREC-12	Female	72	Nephrology	2017/9/18	Urine	MXF+CAZ	Recovered	ST410	X3	50
CREC-13	Male	61	Urology	2017/10/18	Urine	CFP/TZP	Poor	ST167	X3	50
CREC-14	Female	88	Urology	2017/10/23	Sputum	CAZ	Poor	ST167	X3	50
CREC-17	Male	46	Gastroenterology	2017/11/27	Blood	ATM	Poor	ST101	X3	50
CREC-19	Male	73	NICU	2018/2/1	CSF	Untreated	Death	ST167	-	-
CREC-21	Female	62	Hepatology	2018/2/14	Blood	CFP/SUL	Death	ST410	X3	50
CREC-25	Male	59	Rehabilitation	2018/3/7	Urine	Untreated	Recovered	ST359	X3	50
CREC-26	Female	40	Hematology	2018/3/17	Urine	AMK	Recovered	ST38	-	-
CREC-27	Male	83	Gastroenterology	2018/3/20	Pus	CAZ	Recovered	ST101	X3	50
CREC-28	Male	82	Urology	2018/7/8	Urine	Untreated	Recovered	ST167	-	-

Abbreviations: CREC, carbapenem-resistant E. coli; NICU, neurosurgical intensive care unit; CSF, cerebrospinal fluid; ZOX, ceftizoxime; IPM, imipenem; CFP/SUL, cefoperazone/sulbactam; ATM, aztreonam; CAZ, ceftazidime; TZP, piperacillin/tazobactam; MXF, moxifloxacin; CFP/TZP, cefoperazone/tazobactam; AMK, amikacin; Inc, incompatibility.

Table 2 Antimicrobial Susceptibilities Of Additional Drug-Resistant Genes Of bla_NDM-5-Positive E. Coli Strains

Isolate	MIC (µg/mL)											Coresistant Genes
	TZP	CAZ	ATM	GEN	AMK	LVX	IPM	MEM	ETM	TGC	CST
CREC-1	>256	>256	>256	0.5	≤1	>32	32	32	128	<1	1	blaCTX-M-55
CREC-2	>256	>256	>256	>256	>256	>32	32	32	128	<1	2	blaCTX-M-55, blaCTX-M-14, rmtB
CREC-4	>256	>256	>256	64	2	>32	32	32	32	<1	2	blaCTX-M-14
CREC-6	>256	>256	>256	>256	>256	>32	32	64	128	<1	0.5	blaCTX-M-55, blaSHV-12, blaTEM-1, rmtB
CREC-8	>256	>256	>256	1	≤1	>32	8	32	64	<1	2	blaCTX-M-55
CREC-9	>256	>256	>256	>256	>256	>32	128	256	128	<1	2	blaCTX-M-55, blaTEM-1, rmtB
CREC-10	>256	>256	0.5	32	4	>32	64	64	128	<1	0.5	blaTEM-1, blaOXA-1, aac(6ʹ)-Ib-cr
CREC-11	>256	>256	0.5	32	4	>32	64	64	128	<1	0.5	blaTEM-1, blaOXA-1, aac(6ʹ)-Ib-cr
CREC-12	>256	>256	0.5	32	4	>32	64	64	128	<1	0.5	blaTEM-1, blaOXA-1, aac(6ʹ)-Ib-cr
CREC-13	>256	>256	>256	>256	>256	>32	128	256	256	<1	1	blaCTX-M-64, blaCTX-M-14, aac(6ʹ)-Ib-cr
CREC-14	>256	>256	>256	>256	>256	>32	128	256	256	<1	1	blaCTX-M-64, blaCTX-M-14, aac(6ʹ)-Ib-cr
CREC-17	>256	>256	2	≤0.5	≤1	>32	8	32	32	<1	1	-
CREC-19	>256	>256	1	>256	>256	>32	8	32	64	<1	2	blaSHV-12, blaTEM-1, rmtB
CREC-21	>256	>256	>256	64	4	>32	8	32	64	<1	2	blaCTX-M-55, blaOXA-1, aac(6ʹ)-Ib-cr
CREC-25	>256	>256	>256	256	≤1	>32	64	256	128	<1	2	blaCTX-M-55
CREC-26	>256	>256	32	256	2	>32	16	64	64	<1	2	blaCTX-M-14
CREC-27	>256	>256	128	≤0.5	≤1	>32	8	64	64	<1	2	blaCTX-M-14
CREC-28	>256	>256	>256	0.5	≤1	>32	8	32	64	<1	2	aac(6ʹ)-Ib-cr

Abbreviations: CREC, carbapenem-resistant E. coli; MIC, minimal inhibitory concentration; TZP, piperacillin/tazobactam; CAZ, ceftazidime; ATM, aztreonam; GEN, gentamicin; AMK, amikacin; LVX, levofloxacin; IPM, imipenem; MEM, meropenem; ETM, ertapenem; TGC, tigecycline; CST, colistin.

Figure 1 Dendrogram derived from the PFGE patterns of 18 bla_NDM-5-positive E. coli strains.

Figure 2 S1-PFGE and Southern blotting of the transferable plasmids in 15 conjugants. The arrows represent the plasmids where the bla_NDM-5 genes located on.