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Original Research

Virulence and Resistance Determinants of Uropathogenic Escherichia coli Strains Isolated from Pregnant and Non-Pregnant Women from Two States in Mexico

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Pages 295-310 | Published online: 30 Jan 2020

Figures & data

Table 1 Antibiotic Resistance of E. coli Strains Isolates from Urine of Pregnant and Non-Pregnant Women from Sonora and Puebla, Mexico

Table 2 Antibiotic Profile Classification of E. coli Isolates from Urine of Women from Sonora and Puebla, Mexico in Relation to Pregnant and Non-Pregnant Conditions

Figure 1 Multiplex-PCR Banding Patterns of Virulence Genes and Individual PCRcnf of E. coli generated by gel electrophoresis. (A) iucD (512 bp), satA (384 bp), fliC (304 bp) and fimH (210 bp). (B) papGII (562 bp), papGIII (421 bp); vatA (330 bp), vatP (226 bp) and iha (150bp). (C) hlyA (1, 280 bp), satP (880 bp) and papA (641 bp). (D) cnf-1 (3, 100 bp). M. Molecular weight market (100 bp Plus DNA Ladder in A, B and C and 1 kb Plus DNA ladder in D) . Lane C+. Positive control (E. coli CFTO73 in A; E. coli CFTO73 plus E. coli O59I in B and E. coli GAG1 in C and D) . Lines with a number E. coli isolated from the samples. C-. Negative control.

Figure 1 Multiplex-PCR Banding Patterns of Virulence Genes and Individual PCRcnf of E. coli generated by gel electrophoresis. (A) iucD (512 bp), satA (384 bp), fliC (304 bp) and fimH (210 bp). (B) papGII (562 bp), papGIII (421 bp); vatA (330 bp), vatP (226 bp) and iha (150bp). (C) hlyA (1, 280 bp), satP (880 bp) and papA (641 bp). (D) cnf-1 (3, 100 bp). M. Molecular weight market (100 bp Plus DNA Ladder in A, B and C and 1 kb Plus DNA ladder in D) . Lane C+. Positive control (E. coli CFTO73 in A; E. coli CFTO73 plus E. coli O59I in B and E. coli GAG1 in C and D) . Lines with a number E. coli isolated from the samples. C-. Negative control.

Figure 2 Frequency of the Virulence Genes Among 150 E. coli Isolates From Urine of Pregnant and Non-Pregnant Women From Sonora and Puebla, Mexico. The studied genes encoded the following virulence factor: fimH, type 1 pilus adhesin; papG+papA, adhesin and pilin of the type P pili; iha, enterobactin receptor/Irg homologue adhesin; iucD, aerobactin; satA+satP, autotransporter and peptidase regions of the secreted autotransporter toxin; vatA+vatP, autotransporter and peptidase regions of the vacuolating autotransporter toxin; hlyA, α-hemolysin; cnf-1, cytotoxic necrotizing factor; fliC, flagellin. The statistically significance results (p < 0.05) are in asterisk.

Figure 2 Frequency of the Virulence Genes Among 150 E. coli Isolates From Urine of Pregnant and Non-Pregnant Women From Sonora and Puebla, Mexico. The studied genes encoded the following virulence factor: fimH, type 1 pilus adhesin; papG+papA, adhesin and pilin of the type P pili; iha, enterobactin receptor/Irg homologue adhesin; iucD, aerobactin; satA+satP, autotransporter and peptidase regions of the secreted autotransporter toxin; vatA+vatP, autotransporter and peptidase regions of the vacuolating autotransporter toxin; hlyA, α-hemolysin; cnf-1, cytotoxic necrotizing factor; fliC, flagellin. The statistically significance results (p < 0.05) are in asterisk.

Table 3 Distribution and Percentage of Positivity of PAI in E. coli Strains Isolated from Urine of Pregnant and Non-Pregnant Women from Sonora (A) and Puebla (B), Mexico and Between States and Conditions

Table 4 E. coli Strains, Isolated from Urine of Pregnant and Non-Pregnant Women from Sonora and Puebla, Associated to Pathotypes or Clinical Cases by the Serotype to Which They Belong

Table 5 Antibiotic Resistance of E. coli Strains Associated to Pathotypes or Clinical Cases by the Serotype to Which They Belong

Table 6 Virulence Genes of E. coli Strains Associated to Pathotypes or Specific Clinic Cases by the Serotype to Which They Belong

Figure 3 Distribution of phylogenetic group among 150 E. coli strains isolated from urine of women from Sonora and Puebla, Mexico. The statistically significance results (p<0.05) are in asterisk.

Abbreviation: CI, Clade I.

Figure 3 Distribution of phylogenetic group among 150 E. coli strains isolated from urine of women from Sonora and Puebla, Mexico. The statistically significance results (p<0.05) are in asterisk.Abbreviation: CI, Clade I.