Species Diversity, Molecular Characterization, and Antimicrobial Susceptibility of Opportunistic Actinomycetes Isolated from Immunocompromised and Healthy Patients of Markazi Province of Iran

Figures & data

Table 1 Isolation Source, Underlying Disease of Patients, and Phenotypic and Biochemical Features of Actinomycetes Isolated from Clinical Samples

Sample Profile					Biochemical Features											16S rRNA Analysis
Isolates	Location (City)	Origin	Immune Defect	Poly Microbial	Opt. Tm	Urease	Catalase	Pigment Production	Tween 80 Hydrolysis	Nitrate Reduction Ne	Growth Rate (Days)	Lysozyme Resistance	Decomposition of Tyrosine	Decomposition of Xanthene	Decomposition of Hypoxanthine	Similarity (%)^a	Base Pair Differences	Identification
A4	Khomein	Lung/Skin abscess	Cancer	−	35	+	+	N	−	+	<7	−	−	−	−	100	0/860	M. fortuitum
A7	Khomein	Abscess	Diabetic	–	35	+	+	N	−	+	<7	−	−	−	−	100	0/878	M. fortuitum
A9	Arak	Wound	Cancer	–	30	+	+	N	−	+	<7	−	−	−	−	100	0/712	M. fortuitum
A2	Arak	Abscess	AIDS	+	35	–	–	Yellow	+	+	<7	−	−	−	−	100	0/689	M. lehmannii
A3	Arak	Wound	Cancer	+	35	+	+	Orange	+	–	<7	−	−	−	−	99.97	2/951	M. flavescens
A11	Khomein	Foot wound	Diabetic	+	30	+	–	Orange	+	+	<7	−	−	−	−	100	0/1240	M. phocaicum
A12	Arak	Skin abscess	Organ transplant	−/+	35	−	–	N	−	+	<7	−	−	−	−	100	0/920	M. abscessus
A13	Khomein	Dental abscess	Cancer	–	35	–	+	N	−	+	>7	−	−	−	−	100	0/980	M. arupense
A15	Khomein	Foot wound	None	–	35	+	+	N	−	+	<7	−	−	−	−	100	0/860	M. setense
A10	Khomein	Abscess	Surgery	–	35	−	+	White	−	−	<7	+	+	–	–	100	0/648	N. neocaledoniensis
A16	Khomein	Brain abscess	Cancer	+	35	−	+	White	−	−	<7	+	−	−	−	100	0/912	N. cyriacigeorgica
A5	Arak	Wound	AIDS	–	35	−	+	White	−	−	<7	+	−	−	−	100	0/1010	N. mexicana
A14	Khomein	Breast abscess	Cancer	+	35	−	+	White	−	−	<7	+	−	−	−	100	0/912	N. mexicana
A6	Khomein	Breast abscess	Cancer	+	30	−	+	Red	−	−	<7	–	−	−	−	100	0/745	S. heliomycini
A17	Arak	Foot wound	Diabetic	–	30	+	+	Red	−	−	<7	–	−	−	−	100	0/745	S. heliomycini
A1	Khomein	Breast abscess	Cancer	–	35	+	+	White	−	−	<7	+	−	−	−	100	0/800	N. farcinica
A8	Khomein	Foot wound	Diabetic	–	30	−	+	Yellow	−	−	<7	–	−	−	−	100	0/745	A. crystalopoetis

Figure 1 16SrRNA sequence based phylogenetic tree for Iranian actinomycetes isolates and nearest validated species of actinomycetes by using the neighbor-joining method. The figures at each node represent bootstrapping values. The tree was rooted with Gordonia terrae.

Table 2 MIC Range and Susceptibility Pattern of Iranian Actinomycetes Using the Broth Microdilution Method

	Isolates	Rifampicin	Imipenem	Streptomycin	Amikacin	Kanamycin	Ciprofloxacin	Ofloxacin	Levofloxacin	Cefoxitin	Doxycycline
A1	N. farcinica	≥128	≥256	≥256	4	4	≤0.5	≤0.25	≤0.5	32	≥256
A2	M. lehmannii	≥64	≥256	≥128	8	6	≤0.5	≤0.25	≤0.5	32	≥256
A3	M. flavescens	≥128	≥256	≥128	4	4	≤0.25	≤0.5	≤0.5	32	≥256
A4	M. fortuitum	≥256	≥128	≥256	4	4	≤0.5	≤0.5	≤0.5	64	≥128
A5	N. mexicana	≤0.5	≥256	2	2	8	1	2	≤0.5	≥256	4
A6	S. heliomycini	≥64	≥256	≥128	8	4	≤0.5	≤0.25	≤0.5	64	≥128
A7	M. fortuitum	≥128	≥256	≥128	8	4	≤0.25	≤0.5	≤0.5	32	≥128
A8	A. crystalopoetis	≥128	≥256	≥128	4	4	≤0.5	≤0.25	≤0.5	32	≥256
A9	M. fortuitum	≤0.5	≥256	2	2	8	1	2	≤0.5	≥256	4
A10	N. neocaledoniensis	≤0.5	≥128	1	2	8	1	2	≤0.5	≥256	2
A11	M. phocaicum	≥64	≤1	≤3	≥128	≤1	≤1	≥32	≤2	≤1	≥256
A12	M. abscessus	≥128	≤1	≤0.5	≤0.25	≥128	≤0.5	≥128	≤1	≤2	≥256
A13	M. arupense	≥64	≤1	≤0.5	≤0.25	≥64	≤1	≥64	≤1	≤2	≥256
A14	N. mexicana	≥64	≤1	≤0.5	≤0.25	≥64	≤1	≥64	≤1	≤2	≥128
A15	M. setense	≥64	≤1	≤ 3	≥128	≤1	≤1	≥32	≤2	≤1	≥256
A16	N. cyriacigeorgica	≥128	≥256	≥256	4	4	≤0.5	≤0.25	≤0.5	32	≥256
A17	S. heliomycini	≥64	≥256	≥128	8	4	≤0.5	≤0.25	≤0.5	64	≥128