Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of Enteroinvasive Escherichia coli (EIEC) Isolates from Patients with Diarrhea in Ahvaz, Iran

Figures & data

Table 1 Primers Used to Identify Virulence-Associated Genes of EIEC

Gene	Primer Sequence (5´-3´)	Amplicon Size (bp)	Annealing Temperature (°C)	Ref.
ipaH	F-GAAAACCCTCCTGGTCCATCAGG R-GCCGGTCAGCCACCCTCTGAGAGTAC	437	61	[^Citation7]
Sen	F- ATGTGCCTGCTATTATTTAT R- CATAATAATAAGCGGTCAGC	799	53	[^Citation9]
virF	F- TCAGGCAATGAAACTTTGAC R- GGGCTTGATATTCCGATAAGTC	618	58	[^Citation9]
invE	F-CGATAGATGGCGAGAAATTATATCCCG R-CGATCAAGAATCCCTAACAGAAGAATCA	766	59	[^Citation9]
Sat	F-TCAGAAGCTCAGCGAATCATTG R-CCATTATCACCAGTAAAACGCACC	930	58	[^Citation8]
sigA	F- CCGACTTCTCACTTTCTCCCG R- CCATCCAGCTGCATAGTGTTTG	430	58	[^Citation8]
sepA	F- GCAGTGGAAATATGATGCGGC R- TTGTTCAGATCGGAGAAGAACG	794	58	[^Citation8]
Pic	F- ACTGGATCTTAAGGCTCAGGAT R- GACTTAATGTCACTGTTCAGCG	572	58	[^Citation8]
ial	F- CTGGATGGTATGGTGAGG R- GGAGGCCAACAATTATTTCC	320	59	[^Citation9]

Table 2 Locus-Specific PCR Primers Selected for MLVA

Locus	Primer Sequence	Repeat Size (bp)	Annealing Temperature (°C)	Ref.
ms06	F-AAACGGGAGAGCCGGTTATT R-TGTTGGTACAACGGCTCCTG	39	57	[^Citation15]
ms07	F-GTCAGTTCGCCCAGACACAG R-CGGTGTCAGCAAATCCAGAG	39	57	[^Citation15]
ms09	F-GTGCCATCGGGCAAAATTAG R-CCGATAAGGGAGCAGGCTAGT	179	57	[^Citation15]
ms11	F-GAAACAGGCCCAGGCTACAC R-CTGGCGCTGGTTATGGGTAT	96	57	[^Citation15]
ms21	F-GCTGATGGCGAAGGAGAAGA R-GGGAGTATGCGGTCAAAAGC	141	57	[^Citation15]
ms23	F-GCTCCGCTGATTGACTCCTT R-CGGTTGCTCGACCACTAACA	375	57	[^Citation15]
ms32	F-TGAGATTGCCGAAGTGTTGC R- AACTGGCGGCGTTTATCAAG	101	57	[^Citation15]

Table 3 Distribution EIEC Strains According to Seasons of Sampling, Age, Gender and Clinical Symptoms

Strain ID	Sex	Age (Month)	Seasonality	Clinical Symptoms
EIEC 1	Female	0–12	Spring	Watery stool, abdominal pain, vomiting
EIEC 2	Female	0–12	Spring	Watery stool, abdominal pain, fever
EIEC 3	Male	0–12	Summer	Watery stool, vomiting, fever
EIEC 4	Male	13–24	Summer	Watery stool, abdominal pain,
EIEC 5	Male	0–12	Summer	Watery stool, abdominal pain, fever
EIEC 6	Female	49–60	Summer	Watery stool, abdominal pain
EIEC 7	Male	0–12	Summer	Abdominal pain, vomiting
EIEC 8	Female	0–12	Spring	Watery stool
EIEC 9	Male	13–24	Winter	Watery stool, abdominal pain
EIEC 10	Male	0–12	Summer	Watery stool, abdominal pain, vomiting
EIEC 11	Female	49–60	Summer	Watery stool
EIEC 12	Male	13–24	Winter	Abdominal pain, vomiting
EIEC 13	Male	0–12	Spring	Watery stool, fever

Figure 1 UPGMA dendrogram based on MLVA type of isolates in regard to their virulence gene. +: Present, -: Absent.

Figure 2 Spanning tree of virulence genes distribution among different MLVA patterns.

Hosseini Nave H, Mansouri S, Moghadam MT, Moradi M. Virulence gene profile and multilocus variable-number tandem-repeat analysis (MLVA) of enteroinvasive Escherichia coli (EIEC) isolates from patients with diarrhea in Kerman, Iran. Jundishapur J Microbiol. 2016;9:6. doi:10.5812/jjm.33529

 Web of Science ®Google Scholar

Cruz C, Souza M, Serra PT, et al. Virulence factors associated with pediatric shigellosis in Brazilian Amazon. Biomed Res Int. 2014;2014. doi:10.1155/2014/539697

 Web of Science ®Google Scholar

Boisen N, Ruiz-Perez F, Scheutz F, Krogfelt KA, Nataro JP. High prevalence of serine protease autotransporter cytotoxins among strains of enteroaggregative Escherichia coli. Am J Trop Med Hyg. 2009;80(2):294–301. doi:10.4269/ajtmh.2009.80.29419190229

 PubMed Web of Science ®Google Scholar

Gorgé O, Lopez S, Hilaire V, Lisanti O, Ramisse V, Vergnaud G. Selection and validation of a multilocus variable-number tandem-repeat analysis panel for typing Shigella spp. JCM. 2008;46(3):1026–1036. doi:10.1128/JCM.02027-07

 Web of Science ®Google Scholar