Discovery of a Novel Hypervirulent Acinetobacter baumannii Strain in a Case of Community-Acquired Pneumonia

Figures & data

Table 1 Antimicrobial Susceptibility Profile of A. baumannii Strains

	AB5075	ZJ06	ATCC17978	LS01
Ceftazidime	>128^＊	128^＊	4	4
Cefepime	>256^＊	128^＊	4	4
Ciprofloxacin	>32^＊	>32^＊	0.25	0.125
Amikacin	64^＊	>256^＊	1	0.5
Tobramycin	32^＊	>64^＊	1	0.5
Meropenem	32^＊	>32^＊	0.5	0.5
Imipenem	32^＊	>32^＊	0.25	0.25
Ampicillin-sulbactam	128^＊	128^＊	2	2
Piperacillin-tazobactam	>256^＊	>256^＊	8	16
Cefoperazone-sulbactam	128^＊	64^＊	2	2
Trimethoprim/sulfamethoxazole	32^＊	32^＊	4^＊	0.625
Tigecycline	1	2	0.125	0.125
Colistin	0.5	0.5	0.25	0.25

Note: ^＊The strain is resistant to the corresponding antimicrobial according to the CLSI breakpoints (Clinical and Laboratory Standards Institute, 28th Edition).

Figure 1 Growth rate study. Growth rate of AB5075, ZJ06, LS01, and ATCC17978 strains were measured at (A) 37°C, (B) 30°C, (C) 42°C, (D) pH=4.5, (E) pH=5.5, and (F) Zn²⁺. Two-way analysis of variance (ANOVA) was used to assess differences between the means.

Figure 2 G. mellonera killing study. The percent survival of G. mellonera after infection with AB5075, ZJ06, LS01, and ATCC17978 was measured for 72 h after infection. Statistical significance was calculated using log rank test. The survival experiments were repeated three times (n=10 larvae per experimental group), and the most representative curves were presented.

Figure 3 Phylogenetic analysis of the novel A. baumannii strain LS01. (A) K-mer tree; (B) single nuclear polymorphism (SNP) tree.