Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China

Figures & data

Table 1 MICs of the Antibiotics Tested in K. pneumoniae BKP19

Antibiotics	MIC (mg/L)
	BKP19	Interpretive Categories
Cefazolin^a	≥64	R
Ceftriaxone^a	≥64	R
Cefepime^a	≥64	R
Cefoxitin^a	≥64	R
Aztreonam^a	≤1	S
Imipenem^b	>32	R
Meropenem^b	16	R
Amikacin^a	≤2	S
Gentamicin^a	≤1	S
Tobramycin^a	≤1	S
Ciprofloxacin^a	≥4	R
Levofloxacin^a	≥8	R
Tigecycline^b	1	S
Colistin^b	0.25	S

Notes: ^aTested by AST-GN16. ^bTested by Etest method.

Abbreviations: MIC, minimum inhibitory concentration; S, susceptible; R, resistant.

Table 2 Antimicrobial Resistance Genes in K. pneumoniae BKP19

Antimicrobial Resistance Gene	Contig	Identity (%)	Position	Antimicrobial Resistance Category
blaSHV-2	1	100	1835313.1836173	Beta-lactam
oqxA	2	99.06	941409.942584	Quinolone
oqxB	2	99.02	938233.941385	Quinolone
fosA	3	99.76	132010.132429	Fosfomycin
aph(6)-Id	4	100	214820.215656	Aminoglycoside
strA	4	100	214017.214820	Aminoglycoside
blaSHV-11	4	99.88	204854.205714	Beta-lactam
catA1	4	99.55	4680.5339	Phenicol
dfrA5	4	100	210580.211053	Trimethoprim
sul2	4	100	220320.221135	Sulphonamide
blaIMP-4	pIMP-4-BKP19	100	5188.5928	Beta-lactam

Figure 1 Backbone structure of the bla_IMP-4-encoding plasmid pIMP-4-BKP19.

Figure 2 Sequence alignment of the pIMP-4-BKP19 plasmid from Klebsiella pneumoniae strain BKP19 with data in the NCBI GenBank database revealed several highly identical plasmids from different Gram-negative bacillus strains, including pIMP-HZ1 (K. pneumoniae strain Kp1, accession no. KU886034), p24854-IMP (K. pneumoniae strain 24854, accession no. MH909341), pIMP-GZ1058 (Escherichia coli strain CRE1058, accession no. KU051709), pIMP-HK1500 (Citrobacter freundii strain CRE1500, accession no. KT989599), pIMP-SH1506 (Enterobacter cloacae strain CRE1506, accession no. KT989598), p13SP-IMP (Klebsiella pneumoniae strain 13-sp, accession no. MH909334), P378-IMP (Pseudomonas aeruginosa strain P378, accession no. KX711879), and p128379-IMP (Enterobacter hormaechei strain 128379, accession no. MF344559).

Figure 3 Comparative analysis of the homologous regions shared by three IncN-type plasmids (pIMP-4-BKP19, p24854-IMP and pIMP-HZ1). The plasmids were annotated by Rapid Annotation using Subsystem Technology (RAST) server. The figure was produced using EasyFig v. 2.2.3, and BLASTN was used to compare sequence homology with the following threshold parameters: minimum length of 100 bp accompanied by 90% identity. Antimicrobial resistance genes are indicated in red, IS elements are indicated in purple, and all other genes are indicated in blue.