Plasmid-Encoded bla_NDM-5 Gene That Confers High-Level Carbapenem Resistance in Salmonella Typhimurium of Pork Origin

Figures & data

Table 1 The Minimum Inhibitory Concentration (MIC) of High-Resistant Salmonella Typhimurium SH160

Antibiotic Category	Antibiotics	MIC^a (μg/mL)	Transconjugant’s MIC (μg/mL)	MIC Standard^b (μg/mL)			Plasmid Carrying Resistant Gene
				Sensitive (S)	Intermediate (I)	Resistant (R)
Penicillin	Ampicillin	>512	256	≦8	16	≧32
Cephalosporin	Cefotaxime	32	32	≦	2	≧4	blaNDM-5
	Cefepime	16	2	≦2	–	≧16
Aminoglycosides	Gentamicin	128	8	≦4	8	≧16	aac(3)-IV, aadA1, aadA3, aph(3ʹ)-la, aph(4ʹ)-la,
	Streptomycin	16	>1024	–	–	≧64
	Amikacin	8	1	≦16	32	≧64
Quinolones	Ofloxacin	1	0.125	≦2	4	≧8
	Ciprofloxacin	2	0.25	≦0.06	0.12–0.5	≧1
Sulfonamides	Nalidixic acid	>512	64	≦16	–	≧32	sul1, sul2, sul3, dfrA12
	Sulfisoxazole	>512	64	≦256	–	≧512
Chloramphenicols	Florfenicol	64	64	≦4	8	≧16	floR, cmlA
	Chloromycetin	128	4	≦8	16	≧32
Colistin	Polymyxins B	0.5	0.125	≦2	4	≧8
Tetracyclines	Tetracycline	4	4	≦4	8	≧16
Carbapenems	Imipenem	8	4	≦1	2	≧4	blaNDM-5

Notes: ^aThe MIC of Salmonella Typhimurium of SH160. ^bAll MICs were interpreted according to Clinical and Laboratory Standards Institute (CLSI).

Figure 1 S1-PFGE and hybridization indicating the transfer of single plasmid harbouring blaNDM-5. Lane 1, M, H-9812 (PFGE marker strain, S. Braenderup H9812; Lane 2, SH160, S. Typhimurium SH160 (donor); Lane 3, Tr160, EC600 (transconjugant)).

Figure 2 Features of the genetic structure of pNDM5_WCHEC0215 (Genbank accession number KY435936), pNDM_MGR194 (Genbank accession number KF220657), pNDM5_SH160, and RJA274 plasmid (Genbank accession number KF877335). The bla_NDM-1/5 gene is shown in red. IS3000, ISAba125, and other insert sequences are shown in green. The replicons are shown in blue. The rest of the genes are shown in orange.