Effect of Short-Term Antimicrobial Therapy on the Tolerance and Antibiotic Resistance of Multidrug-Resistant Staphylococcus capitis

Figures & data

Figure 1 Overview of the antibacterial regimen and strain isolation.

Table 1 Antimicrobial Susceptibility of the S. capitis Isolates

Strain ID	Sca70935	Sca71187	Sca71207	Resistance Gene*
PEN	≥0.5(R)	≥0.5(R)	≥0.5(R)	blaZ
OXA	≥4(R)	≥4(R)	≥4(R)	mecA
ERY	≥8(R)	≥8(R)	≥8(R)	ErmA
LIN	≥8(R)	≥8(R)	≥8(R)
SXT	≤10(S)	≤10(S)	≤10(S)	dfrC
QDA	4(R)	2(I)	2(I)
LNZ	≥8	≥8	≥8	cfrA
GEN	≥16(R)	4(S)	4(S)	aac(6ʹ)-Ie,ant(4ʹ)-Ib ^Δ
TGC	0.5(S)	0.25(S)	0.25(S)
TET	4(S)	2(S)	2(S)
NIT	≤16(S)	≤16(S)	≤16(S)
RIF	≤0.5(S)	≥64(R)	≥64(R)	rpoB^#
VAN	≤0.5(S)	≤0.5(S)	≤0.5(S)
TEC	1(s)	1(s)	1(s)
LEV	8(R)	8(R)	8(R)	qacA
MFX	4(R)	4(R)	4(R)	norA, mgrA
CIP	≥8(R)	≥8(R)	≥8(R)
CIR	Neg	Neg	Neg

Notes: *The three strains carry the same resistance gene. ^#rpoB mutation was identified in strains Sca71187 and Sca71187. ^Δaac(6′)-Ie and ant(4′)-Ib are aminoglycan-resistance genes, but mainly result in amikacin resistance.

Abbreviations: PEN, penicillin G; OXA, oxacillin; ERY, erythromycin; LIN, lindamycin; SXT, trimethoprim/sulfamethoxazole; QDA, quinupristin/dalofopine; LNZ, linezolid; GEN, gentamicin; TGC, tigecycline; TET, tetracycline; NIT, nitrofurantoin; RIF, rifampin; VAN, vancomycin; TEC, teicoplanin; LEV, levofloxacin; MFX, moxifloxacin; CIP, ciprofloxacin; CIR, clindamycin-induced resistance.

Table 2 Summary of the Protein Variants in the Sca71187 and Sca71207 Strains

ID	SaeR	MoaA	rpoB	hp
Sca71187	c.43A>G p.Asn15Asp	c.389T>C p.Val130Ala	c.1430C>A p.Ala477Asp	frameshift_variant c.552delA p.Lys184fs
Sca71207	c.43A>G p.Asn15Asp	c.389T>C p.Val130Ala	c.1430C>A p.Ala477Asp	frameshift_variant c.552delA p.Lys184fs

Abbreviations: hp, hypothetical protein; c., mutation of nucleic acid; p., change of coding protein.

Figure 2 Predictive analysis of genes with unknown function. (A) Structure prediction of a hypothetical protein based on the amino acid sequence. (B) Structure of a homologous protein from the SWISS-MODEL database (SEQ ID NO: 5uvd.1.A). (C) Comparison of the proteins shown in panels (A and B) proteins. Blue, identical amino acids in both proteins; gray, different amino acids. (D) Possible cellular distribution of the hypothetical protein.

Figure 3 (A) Growth curve. (B) Biofilm formation. (C) In vivo infection of G. mellonella larvae. (D) survival of bacteria in whole blood *** indicated (p<0.001).