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Figures & data
Table 1 Primers Used for Multiplex PCR Detection of Mcr Genes
Figure 1 Multiplex PCR detection of mcr genes. Purified plasmid pUC57 carrying each mcr gene was used as templates. Agarose gel electrophoresis (2.0%) was used to separate multiplex PCR products. M indicates the molecular size marker (Trans100bp plus DNA ladder, TransGen Biotech, China). The size of each amplicon is indicated on the right side. NC, empty vector pUC57 was included as negative control.
Figure 2 Multiplex PCR detection of two (A) or three (B) mcr genes. Different mixtures of double or triple plasmid DNA carrying a single mcr gene were used as templates. M indicates the molecular size marker.
Figure 3 Molecular detection of mcr genes in bacterial isolates by the multiplex PCR method. Colistin-resistant bacterial strains (43 isolates of E. coli and 17 isolates of K. pneumoniae) were tested for the presence of mcr genes. Total bacterial DNA was obtained by the boiling method and applied to the multiplex PCR assay. E. coli strain Top10 harboring the mcr-encoding plasmid was included as a positive control. NC, E. coli TOP10 with empty vector pUC57. M shows the molecular size marker.
