https://orcid.org/0000-0002-6714-547X
Figures & data
Table 1 The Sequence of Primers Used in the RT-PCR Amplification
Figure 1 Effect of sub-MICs of tyrosol (A) and EDTA (B) on quorum-sensing signals molecules using reporter strain assay.
Notes: All used sub-MICs of tyrosol and EDTA caused a significant reduction in the C12-HSL signal. Tyrosol and EDTA sub-MICs (1/4x, 1/8x, and 1/16x MIC) caused significant reduction in the C4-HSL signal. *P< 0.05, ***P< 0.001.
Abbreviations: MIC, minimum inhibitory concentration; HSL, homoserine lactone.
Figure 2 Effect of sub-MICs of tyrosol (A) and EDTA (B) on the production of lipase, protease, and lecithinase enzymes of PAO1.
Notes: Tyrosol and EDTA (1/4xMIC) caused a significant reduction in Protease and lipase enzymes’ production. Only EDTA sub-MICs (1/4x and1/8x MIC) caused a significant reduction in lecithinase production. *P< 0.05, ***P< 0.001.
Abbreviations: MIC, minimum inhibitory concentration; HSL, homoserine lactone.
Table 2 Comparison of the Swimming, Swarming and Twitching Motilities Which Were Shown by PAO1 and PAO-JP2 and Treated PAO1 with Sub-MICs of Tyrosol and EDTA
Figure 3 Effect of sub-MICs (1/4x and 1/8x MIC) of tyrosol and EDTA on the level of expression of QS regulatory genes: (A) relative expression of lasI/lasR, (B) relative expression of rhlI/rhIR and (C) relative expression of pqsA/pqsR of treated P. aeruginosa PAO1.
Note: ***P<0.0001.
Abbreviations: MIC, minimum inhibitory concentration; QS, quorum sensing.
