Genetic Diversity of Schistosoma haematobium in Qena Governorate, Upper Egypt

Figures & data

Figure 1 Egypt’s map displaying the location of the Qena governorate, and the red box shows the site of four studied localities in Qena where the participants live (Alhujyrat, Altramsa, Dandara, and Qena).

Table 1 No. of the Included Participants in the Study and Their Distribution

Residence	No. of Included Participants	Percent [%]
Al hujyrat	17	12.7
Al tramsa	11	8.2
Dandara	53	39.5
Qena	53	39.5
Total	134	100.0

Table 2 Criteria of Patients Gave Positive RAPD –PCR Results

Patient No.	Residence	Age	Complaint
7	Dandara	17	Haematuria
9	Qena	5.5	Haematuria
11	Qena	8	Haematuria
13	Al hujirat	23	Haematuria + dysuria
15	Qena	50	Haematuria
17	Qena	40	Haematuria + dysuria
21	Al hujirat	27	Haematuria
22	Qena	46	Haematuria
29	Dandara	2	Haematuria
30	Qena	22	Haematuria
31	Dandara	27	Haematuria
34	Dandara	47	Haematuria
35	Dandara	30	Haematuria
36	Al hujirat	25	Haematuria
37	Dandara	28	Haematuria
38	Al tramsa	8	Haematuria
42	Qena	50	Haematuria
44	Qena	60	Haematuria
45	Dandara	2	Haematuria
47	Dandara	25	Haematuria

Table 3 RAPD Polymorphism Among Human Schistosoma haematobium Isolates

Primer	Total Number of Bands (a)	Amplified Fragment Size Range (bp)	Number of Polymorphic Bands (b)	Polymorphism b/a %
A02	9	1190–223	9	100
A07	9	1395–234	9	100
A09	12	1747–198	12	100
A10	10	1860–241	10	100
Total	40	1860–198	40	100

Figure 2 RAPD-PCR patterns produced from the genomic DNA of Schistosoma haematobium isolated from 20 infected patients generated by four oligoprimers. (A) Positive control. (B) Negative control. (C) DNA Pattern generated with Primer A02. (D) DNA Pattern generated with Primer A07. (E) DNA Pattern generated with Primer A09. (F) DNA pattern with Primer A10. Bands were visualized by 1.2% agarose gel electrophoresis stained with ethidium bromide.

Table 4 Genetic Similarity Coefficient Among Human S. haematobium Isolates Showing Dissimilarities Between Isolates

Similarity Matrix
	H7	H9	H11	H13	H15	H17	H21	H22	H29	H30	H31	H34	H35	H36	H37	H38	H42	H44	H45	H47
H7	1
H9	0.757	1
H11	0.85	0.878	1
H13	0.667	0.71	0.647	1
H15	0.718	0.95	0.837	0.667	1
H17	0.585	0.524	0.622	0.457	0.591	1
H21	0.684	0.769	0.81	0.688	0.78	0.651	1
H22	0.564	0.55	0.605	0.485	0.524	0.682	0.585	1
H29	0.706	0.8	0.737	0.714	0.811	0.564	0.778	0.595	1
H30	0.593	0.5	0.516	0.762	0.467	0.375	0.552	0.4	0.56	1
H31	0.667	0.647	0.649	0.815	0.611	0.579	0.8	0.556	0.581	0.667	1
H34	0.667	0.571	0.581	0.667	0.533	0.313	0.483	0.333	0.48	0.667	0.583	1
H35	0.424	0.353	0.378	0.296	0.333	0.474	0.4	0.611	0.258	0.25	0.467	0.333	1
H36	0.615	0.519	0.533	0.8	0.483	0.387	0.571	0.414	0.583	0.824	0.696	0.824	0.261	1
H37	0.813	0.727	0.722	0.692	0.686	0.486	0.647	0.4	0.533	0.609	0.69	0.696	0.345	0.636	1
H38	0.545	0.471	0.486	0.593	0.444	0.474	0.514	0.611	0.516	0.583	0.6	0.5	0.4	0.609	0.552	1
H42	0.462	0.519	0.467	0.4	0.483	0.323	0.429	0.414	0.333	0.353	0.435	0.471	0.348	0.375	0.545	0.435	1
H44	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1
H45	0.286	0.273	0.24	0.4	0.25	0.231	0.261	0.167	0.316	0.333	0.333	0.5	0	0.545	0.353	0.333	0.364	0	1
H47	0.643	0.552	0.563	0.727	0.516	0.364	0.533	0.387	0.538	0.737	0.64	0.842	0.32	0.889	0.667	0.64	0.444	0	0.462	1

Figure 3 Dendrogram (UPGMA) based on Nei and Li similarity coefficient.