Figures & data
Table 1 Primer Sequences for the Confirmation of Diarrheagenic E. coli and Salmonella Isolates
Table 2 A Panel of Commercial Antimicrobial Disc Used for Susceptibility Testing of Diarrheagenic E. coli Isolates
Table 3 A Panel of Commercial Antimicrobial Disc Used for Susceptibility Testing of Salmonella Isolates
Table 4 Oligonucleotides Sequences for the Detection of Resistance Gene Determinants
Figure 1 Representative Gel electrophoresis image of the confirmed DEC isolates recovered from diarrheagenic stool specimens. Lane (M) Molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: negative control, lane 2 to 12: Some of the positive diarrheagenic E. coli isolates.
![Figure 1 Representative Gel electrophoresis image of the confirmed DEC isolates recovered from diarrheagenic stool specimens. Lane (M) Molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: negative control, lane 2 to 12: Some of the positive diarrheagenic E. coli isolates.](/cms/asset/b27076f4-b007-4933-aa91-279a9c441931/didr_a_269219_f0001_b.jpg)
Figure 2 Representative Gel electrophoresis image of the confirmed Salmonella isolates recovered from diarrheagenic stool specimens. Lane (M) Molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: positive control S. enterica subsp. Typhimurium (DSMZ 14028), lane 2: negative control, lane 3 to 13: Some of the positive Salmonella isolates.
![Figure 2 Representative Gel electrophoresis image of the confirmed Salmonella isolates recovered from diarrheagenic stool specimens. Lane (M) Molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: positive control S. enterica subsp. Typhimurium (DSMZ 14028), lane 2: negative control, lane 3 to 13: Some of the positive Salmonella isolates.](/cms/asset/17e84909-ff84-44bd-a6c2-3827944bfa0d/didr_a_269219_f0002_b.jpg)
Table 5 Patterns of Multiple Antibiotic-Resistance Phenotypes (MARPs) of Diarrheagenic E. coli
Table 6 Patterns of Multiple Antibiotic-Resistance Phenotypes (MARPs) of Salmonella Isolates
Figure 3 Gel electrophoresis representation of β-Lactam resistance genes ampC (198 bp) detected in DEC and Salmonella isolates. Lane (M) Molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: negative control, lane 2 to 13: some of the representatives of the genetic expression of ampC (198 bp) from the β- Lactams -resistant DEC and Salmonella isolates.
![Figure 3 Gel electrophoresis representation of β-Lactam resistance genes ampC (198 bp) detected in DEC and Salmonella isolates. Lane (M) Molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: negative control, lane 2 to 13: some of the representatives of the genetic expression of ampC (198 bp) from the β- Lactams -resistant DEC and Salmonella isolates.](/cms/asset/3059698b-d090-4789-a691-9b79fffae8d0/didr_a_269219_f0003_b.jpg)
Figure 4 A representative gel electrophoresis profile of some β- Lactam resistant genes detected in DEC and Salmonella isolates. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: negative control, lane 2 to 13: some of the representatives of the genetic expression of blaTEM (690 bp) from the β-Lactams -resistant DEC.
![Figure 4 A representative gel electrophoresis profile of some β- Lactam resistant genes detected in DEC and Salmonella isolates. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo Scientific), lane 1: negative control, lane 2 to 13: some of the representatives of the genetic expression of blaTEM (690 bp) from the β-Lactams -resistant DEC.](/cms/asset/74beb83f-db09-4c25-a867-37924747b6b6/didr_a_269219_f0004_b.jpg)
Figure 5 An illustrative gel electrophoresis image with the genetic expression of the aadA (525 bp) resistant gene among the aminoglycosides-resistant DEC and Salmonella isolates. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), lane 1: negative control, lane 2 to 11: some of the representatives of the genetic expression of aadA (525 bp) from aminoglycoside-resistant DEC and Salmonella isolates.
![Figure 5 An illustrative gel electrophoresis image with the genetic expression of the aadA (525 bp) resistant gene among the aminoglycosides-resistant DEC and Salmonella isolates. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), lane 1: negative control, lane 2 to 11: some of the representatives of the genetic expression of aadA (525 bp) from aminoglycoside-resistant DEC and Salmonella isolates.](/cms/asset/9405f751-f827-42e3-9b84-f5e311e9e039/didr_a_269219_f0005_b.jpg)
Figure 6 An illustrative gel electrophoresis image for the detection of sulI (822 bp) resistance gene from the sulfonamide-resistant DEC. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), lane 1: negative control, lane 2 to 13: some of the representatives of the genetic expression of sulI (822 bp) from the sulfonamide-resistant DEC.
![Figure 6 An illustrative gel electrophoresis image for the detection of sulI (822 bp) resistance gene from the sulfonamide-resistant DEC. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), lane 1: negative control, lane 2 to 13: some of the representatives of the genetic expression of sulI (822 bp) from the sulfonamide-resistant DEC.](/cms/asset/d4eb78f0-2fc6-484d-acdd-a6ec5d9b884f/didr_a_269219_f0006_b.jpg)
Figure 7 An illustrative gel electrophoresis for the detection of sulII (625 bp) resistance gene. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), lane 1: negative control, lane 2 to 10: some of the representatives of the genetic expression of sulII (625 bp) from the sulfonamide-resistant DEC and Salmonella isolates.
![Figure 7 An illustrative gel electrophoresis for the detection of sulII (625 bp) resistance gene. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), lane 1: negative control, lane 2 to 10: some of the representatives of the genetic expression of sulII (625 bp) from the sulfonamide-resistant DEC and Salmonella isolates.](/cms/asset/2ff3d896-9c73-4b36-9e21-c701762d84fe/didr_a_269219_f0007_b.jpg)
Figure 8 A representative gel electrophoresis image for the detection tet A (201 bp) resistance gene determinants. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), Lane 1: negative control, Lane 2 to 8: some of the representatives of the genetic expression of tet A (201 bp) from the Tetracycline-resistant DEC isolates.
![Figure 8 A representative gel electrophoresis image for the detection tet A (201 bp) resistance gene determinants. Lane (M) molecular weight marker (100 bp DNA ladder, Thermo scientific), Lane 1: negative control, Lane 2 to 8: some of the representatives of the genetic expression of tet A (201 bp) from the Tetracycline-resistant DEC isolates.](/cms/asset/00997f61-cfe4-4000-ad6a-7a1e2e76ad14/didr_a_269219_f0008_b.jpg)