The Frequency of Natural Killer Cell Subsets in Patients with Acquired Immune Deficiency Syndrome with Deep Fungal Infections

Figures & data

Table 1 Comparison of Basic Information and Blood Tests Among the Three Groups (±s)

Index	HIV + IFI Group (n=390)	HIV+Non-IFI Group (n=439)	Control Group (n=200)	Statistical Value	P value
Male/female	300/90	332/107	160/40	1.483	0.476
Age	36.12±11.56	35.34±11.58	36.89±11.63	3.015	0.072
HIV RNA (log10 copies/mL)	5.94±2.69	5.28±1.96	Undetectable	2.871	0.282
Leucocyte (1/L)	4259±176.46	5832±173.48	6012±174.32	5428.28	<0.0001
Lymphocyte (1/μL)	589.76±67.65	1986.87±95.42	2196±100.12	17,549.48	<0.0001
CD4^+ T cells (1/μL)	13.24±7.13	402.57±42.43	748.23±102.35	134.03	<0.0001
Proportion of CD4^+T cells in total lymphocytes (%)	2.15±1.43	22.79±6.38	36.78±6.89	43.08	<0.0001
CD8^+ T cells (1/μL)	299.15±33.74	690.54±64.99	622.56±203.48	69.58	<0.0001
Proportion of CD8^+T cells in total lymphocytes (%)	59.18±14.98	37.47±9.46	28.92±8.82	19.02	<0.0001
CD4^+ T cells/CD8^+ T cells	0.04±0.02	0.71±0.06	1.14±0.36	160.14	<0.0001

Figure 1 Flow cytometry results (A–C).

Table 2 Distribution of NK Cell Subsets in Three Groups of Patients (±s)

Index	HIV + IFI Group (n=390)	HIV+Non-IFI Group (n=439)	Control Group (n=200)	Statistical Value	P value
NK cells (1/μL)	88.56±17.32	330.12±30.12	319.65±28.56	5479.13	<0.0001
Proportion of NK cells in total lymphocytes (%)	13.12±4.98	17.02±6.57	14.34±3.57	28.15	<0.0001
CD56^brightNKcells (1/μL)	4.45±1.56	12.23±4.58	13.23±3.58	298.68	<0.0001
Proportion of CD56^brightNKcells in NK cells (%)	5.32±1.41	4.45±1.45	4.02±1.32	30.53	0.001
CD56^dimNKcells (1/μL)	37.13±10.60	227.32±50.61	253.37±11.60	998.72	<0.0001
Proportion of CD56^dimNKcells in NK cells (%)	65.23±5.41	82.03±8.31	79.32±3.61	543.73	<0.0001
CD56^−CD16^+ NK cells (1/μL)	25.76±8.97	43.12±9.62	51.12±8.62	265.53	<0.0001
Proportion of CD56^−CD16^+ NK cells in NK cells (%)	33.12±7.63	15.52±3.44	15.78±4.74	537.68	<0.0001

Table 3 Analysis of the Correlation Between T Cell and NK Cell Subset Distribution and HIV-RNA Expression

Cell Subset	B	SE	Wald	OR Value	95% CI	p-value	HIV + IFI Group (n=390)	HIV+Non-IFI Group (n=439)	Control Group (n=200)
CD4^+T cell	−0.288	0.104	4.697	0.624	0.602~0.629	0.018	13.24±7.13	402.57±42.43	748.23±102.35
CD8^+T cell	0.104	0.619	1.579	0.134	0.009~1.995	0.155	299.15±33.74	690.54±64.99	622.56±203.48
CD4^+T cell/CD8^+T cell	−0.378	0.115	1.246	0.675	0.643~0.709	0.001
CD56^brightNK cell	−0.147	0.776	1.89	0.142	0.015~1.344	0.086	4.45±1.56	12.23±4.58	13.23±3.58
CD56^dimNK cell	−0.363	0.113	4.479	0.362	0.303~0.411	0.351	37.13±10.60	227.32±50.61	253.37±11.60
CD56^−CD16^+NK cell	−0.195	0.617	1.352	0.371	0.105~1.299	0.122	25.76±8.97	43.12±9.62	51.12±8.62
NK cell	−0.372	0.107	4.866	0.683	0.492~0.973	0.037	88.56±17.32	330.12±30.12	319.65±28.56