Multiple Expression Assessments of ACE2 and TMPRSS2 SARS-CoV-2 Entry Molecules in the Urinary Tract and Their Associations with Clinical Manifestations of COVID-19

Figures & data

Figure 1 The expression of ACE2 and TMPRSS2 in tissue.

Notes: (A) The mRNA and protein expression pattern of ACE2 in HPA, GTEx and FANTOM5. (B) The mRNA and protein expression pattern of TMPRSS2 in HPA, GTEx and FANTOM5. (C) The mRNA expression pattern of ACE2 and TMPRSS2 in GSE3526. (D) The mRNA expression pattern of ACE2 and TMPRSS2 in GSE8124. (E) The mRNA expression pattern of ACE2 and TMPRSS2 in central nervous system. (F) The mRNA expression pattern in TCGA, ** = P<0.01, **** = P<0.0001, ns = P>0.05. (G) The mRNA expression pattern of TMPRSS2 in TCGA, ** = P<0.01, **** = P<0.0001, ns = P>0.05.

Figure 2 The panel of immunohistochemical image of ACE2 and TMPRSS2 in target organs.

Notes: (A) The panel of immunohistochemical images of ACE2 in kidney. (B) The panel of immunohistochemical images of ACE2 in testis. (C) The panel of immunohistochemical images of TMPRSS2 in kidney. (D) The panel of immunohistochemical images of TMPRSS2 in prostate.

Figure 3 ACE2 and TMPRSS2 expression patterns in specific patient populations.

Notes: (A) ACE2 and TMPRSS2 expression patterns in GSE113439, * = P<0.05, ** = P<0.01, *** = P<0.001, ns = P>0.05. (B) ACE2 and TMPRSS2 expression patterns in GSE66494, * = P<0.05, ** = P<0.01, *** = P<0.001, ns = P>0.05. (C) ACE2 and TMPRSS2 expression patterns in GSE96804, * = P<0.05, ** = P<0.01, *** = P<0.001, ns = P>0.05. (D) ACE2 and TMPRSS2 expression patterns in GSE10006, * = P<0.05, ** = P<0.01, *** = P<0.001, ns = P>0.05. (E) ACE2 and TMPRSS2 expression patterns in female. (F) ACE2 and TMPRSS2 expression patterns in male.

Table 1 The Brief Review of Existing Paper with Genitourinary System Impairment

Journal	Author (Year)	Cases			Event
		Total	Male	Female
Lancet	Huang et al (2020)^Citation8	41	30	11	AKI: 3; CRRT: 3
JAMA	Wang et al (2020)^Citation10	138	75	63	AKI: 3; CRRT: 2
Lancet	Zhou et al (2020)^Citation9	191	119	72	AKI: 27; CRRT:10
Intensive Care Medicine	Ruan et al (2020)^Citation11	150	102	48	AKI:21; CRRT: 5
BMJ	Chen et al (2020)^Citation12	274	171	103	AKI: 28; CRRT: 3
NEJM	Guan, et al (2020)^Citation13	1099	637	459	AKI: 5; CRRT: 9
Intensive Care Med	Tu et al (2020)^Citation14	174	79	95	AKI: 19; CRRT: 7
Intensive Care Med	Cao, et al (2020)^Citation15	102	53	49	AKI: 8; CRRT:4
Fertility and Sterility	Pan et al (2020)^Citation16	34	34	0	Scrotal discomfort: 19
JAMA	Richardson et al (2020)^Citation17	5700	3437	2263	AKI: 523; CRRT: 81
J Am Soc Nephrol	Pei et al (2020)^Citation18	333	182	151	AKI: 35
JAMA Netw Open	Li et al (2020)^Citation19	38	38	0	SARS-CoV-2 positive in semen: 6

Abbreviations: AKI, acute kidney injury; CRRT, continuous renal replacement therapy.

Table 2 The Pairwise Distance of ACE2 in Different Species

	1	2	3	4	5	6	7	8	9	10	11	12
Homo sapiens	–
Tadorna cana	0.434
Felis catus	0.146	0.421
Mustela putorius furo	0.160	0.418	0.093
Canis lupus familiar	0.144	0.426	0.078	0.082
Gallus gallus	0.427	0.122	0.422	0.416	0.427
Sus scrofa	0.172	0.438	0.140	0.152	0.150	0.440
Procyon lotor	0.154	0.428	0.093	0.063	0.078	0.422	0.161
Paguma larvata	0.164	0.419	0.060	0.108	0.097	0.425	0.159	0.108
Panthera tigris altaica	0.144	0.424	0.010	0.091	0.077	0.421	0.143	0.091	0.060
Bos taurus	0.166	0.440	0.141	0.152	0.139	0.437	0.121	0.148	0.152	0.138
Nyctereutes procyonoides	0.142	0.423	0.075	0.079	0.009	0.424	0.150	0.077	0.094	0.075	0.138	–

Abbreviations: Homo sapiens, human; Gallus gallus, domestic chicks; Tadorna cana, waterfowl; Felis catus, cats; Mustela Putorius furo, domestic ferrets; Sus scrofa, wild boars; Canis lupus familiar, dogs; Procyon lotor, raccoon; Paguma larvata, masked palm civets; Panthera tigris altaica, tigers; Bos taurus taurus, domestic cattle; Nyctereutes procyonoides, raccoon dogs.

Figure 4 Multiple sequence alignment of ACE2.

Figure 5 Cell-specific expression of ACE2 and cytokine receptors in kidney.

Notes: (A) Cell-specific expression of ACE2 from http://humphreyslab.com/SingleCell/(Healthy adult human kidney, epithelia). (B) Cell-specific expression of ACE2 from http://humphreyslab.com/SingleCell/(Healthy adult human kidney, complete). (C) Cell-specific expression of ACE2 and cytokine receptors from Human Cell Landscape Kidney2 (http://bis.zju.edu.cn/HCL/search.html). (D) Cell-specific expression of ACE2 and cytokine receptors from Human Cell Landscape Kidney3 (http://bis.zju.edu.cn/HCL/search.html). (E) Cell-specific expression of ACE2 and cytokine receptors from Human Cell Landscape Kidney4 (http://bis.zju.edu.cn/HCL/search.html).

Figure 6 Cell-specific expression of ACE2 and cytokine receptors in testis.

Notes: (A) Cell-specific expression of ACE2 in GSE124263. (B) Cell-specific expression of ACE2 and cytokine receptors from Human Cell Landscape http://bis.zju.edu.cn/HCL/search.html.

Figure 7 GSEA of ACE2-positive cells in kidney and testis.

Notes: (A) GSEA of ACE2-positive cells in kidney (kidney proximal tubule cells). (B) GSEA of ACE2-positive cells in testis (spermatogonial stem cells, Leydig cells, Sertoli cells, and myoid cells).

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