A Multidrug-resistant Monophasic Salmonella Typhimurium Co-harboring mcr-1, fosA3, bla_CTX-M-14 in a Transferable IncHI2 Plasmid from a Healthy Catering Worker in China

Figures & data

Table 1 The Minimum Inhibitory Concentration (MIC) Values of the Donor (Salmonella 4,[5],12:i:- YZU1189), Transconjugant (E. coli C600-pYZU1189) and Recipient (E. coli C600)

Classes	Antibiotics	MIC (mg/L)			Antibiotic Resistance Genes (YZU1189)
		YZU1189 (S. 4,[5],12:i:-)	C600 (E. coli)	C600-pYZU1189 (E. coli)
Aminoglycosides	Gentamicin	16 (R)	0.25 (S)	64 (R)	aac(3)-IV, aph(4)-Ia, aph(3ʹ)-Ia, aac(6ʹ)-Iaa
	Streptomycin	4 (S)	>256 (R)	>256 (R)
	Amikacin	0.5 (S)	1 (S)	2 (S)
Beta-lactams	Ampicillin	>128 (R)	2 (S)	>128 (R)	blaCTX-M-14
	Cefazolin	>128 (R)	2 (S)	>128 (R)
	Cefotaxime	32 (R)	0.06 (S)	4 (R)
Carbapenems	Meropenem	0.015 (S)	0.03 (S)	0.03 (S)
Polymyxins	Colistin	8 (R)	0.25 (S)	8 (R)	mcr-1
Fosfomycins	Fosfomycin	>512 (R)	32 (S)	>512 (R)	fosA3
Phenicols	Chloramphenicol	128 (R)	2 (S)	64 (R)	floR
	Florfenicol	>128 (R)	4 (S)	128 (R)
Trimethoprims /sulfonamides	Trimethoprim/ sulfamethoxazole	64 (R)	1 (S)	64 (R)	sul2, sul3, drfA12
Tetracyclines	Tetracycline	128 (R)	2 (S)	2 (S)	tet(B)
Fluoroquinolones	Ciprofloxacin	1 (S)	0.015 (S)	0.015 (S)	gyrA(D87N)
	Nalidixic acid	>256 (R)	4 (S)	4 (S)

Abbreviations: MIC, minimum inhibitory concentration; R, resistant; S, susceptible.

Figure 1 Sequence alignment of pYZU1189 and pHNSHP45-2 (GenBank no. KU341381). The pHNSHP45-2 was used as a reference to compare with mcr-1-bearing plasmid which possess the IncHI2 replicon in this study. Red arrows represent the plasmid pHNSHP45-2.