First Full-Length Genome Sequence of Dengue Virus Serotype 2 Circulating in Vietnam in 2017

Figures & data

Table 1 Genetic Variations in the Dengue Type 2 Genome Circulated in Dengue Epidemic of 2017 in Vietnam. Description of Amino Acid Substitution in the 7 DENV-2 Virus Compared to the 10 Reference Strains. Homologous Amino Acids are Denoted by (-), and the Modified Amino Acids are Denoted by Letters. The Amino Acid Site Including Replacement of Hydrophilic and Hydrophobic are Listed in Each Gene

AA Variants Gene	AA Position (Protein)	7 Dengue Strains in this Study	Vietnam 2004 EU482774	Vietnam 2008 FJ410215	Thailand 2001 FJ639832	Malaysia 2008 FJ467493	Indonesia 1998 AB189124	India 2006 FJ898454	China 2013 MF459663	Cambodia 2008 GU131902	Brunei 2005 EU179858	Brazil 2006 FJ850088
C	76	1(R)/6(K)	K	–	–	–	–	–	–	–	–	–
PrM	0	0
E	120	1(I)/6(T)	T	–	–	–	–	–	–	–	–	–
NS1	156	1(G)/6(S)	N	–	–	S	S	S	–	–	S	S
NS2A	91	2(T)/5(S)	S	–	–	–	–	–	–	–	–	–
	106	1(I)/4(V)	V	–	–	–	I	I	–	–	I	I
NS2B	0	0
NS3	133	1(G)/6(D)	D	–	–	–	–	–	–	–	–	–
NS4A	71	1(G)/6(S)	S	–	–	–	–	–	–	–	–	–
NS4B	0	0
NS5	258	2(L)/5(I)	L	–	–	I	I	I	–	–	I	V
	260	2(T)/5(M)	T	–	–	M	M	M	–	–	M	M
	639	2(I)/5(L)	L	–	–	–	–	–	–	–	–	–
	683	1(Q)/6(H)	C	–	–	–	–	–	–	–	–	–
	684	1(M)/6(I)	M	–	–	–	–	–	–	–	–	–
	687	1(L)/6(V)	L	–	–	–	–	–	–	–	–	–

Figure 1 Genomic clustering of 7 DENV2 samples and 49 reference sequences based on MASH distances. Heatmap illustrates the pairwise similarity between samples, color scale ranges from 0 (blue) to 0.1 (red). Two large groups are identified and colored with the same key. The MASH clustering also reflects the significant difference between references from Asia and from other continents when comparing with the individual samples in this study.

Figure 2 Phylogenetic tree based on the level of a similar sequence of 7 DENV2 strains and 49 reference viruses. The viruses from Vietnam in 2017 are denoted by D2, the strains circulated in Vietnam in the past and in the world are denoted by the country name, GenBank accession number and the year causing the disease.