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Infection and Drug Resistance Volume 14, 2021 - Issue
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Original Research

Detection of gyrA and parC Mutations and Prevalence of Plasmid-Mediated Quinolone Resistance Genes in Klebsiella pneumoniae

Sawsan Mohammed Kareem1 Branch of Biotechnology, Department of Biology, College of Science, University of Mustansiriyah, Baghdad, Iraq
,
Israa M S Al-kadmy1 Branch of Biotechnology, Department of Biology, College of Science, University of Mustansiriyah, Baghdad, Iraq;2 Faculty of Science & Engineering, School of Engineering, University of Plymouth, Plymouth, PL4 8AA, UKCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-5109-7338
ORCID Icon,
Saba S Kazaal1 Branch of Biotechnology, Department of Biology, College of Science, University of Mustansiriyah, Baghdad, IraqORCID Iconhttps://orcid.org/0000-0003-4975-2660
ORCID Icon,
Alaa N Mohammed Ali1 Branch of Biotechnology, Department of Biology, College of Science, University of Mustansiriyah, Baghdad, Iraq
,
Sarah Naji Aziz1 Branch of Biotechnology, Department of Biology, College of Science, University of Mustansiriyah, Baghdad, Iraq
,
Rabab R Makharita3 Botany Department, Faculty of Science, Suez Canal University, Ismailia, 41522, EgyptORCID Iconhttps://orcid.org/0000-0002-6430-7541
ORCID Icon,
Abdelazeem M Algammal4 Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, EgyptORCID Iconhttps://orcid.org/0000-0001-5580-1559
ORCID Icon,
Salim Al-Rejaie5 Department of Pharmacology & Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
,
Tapan Behl6 Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, IndiaORCID Iconhttps://orcid.org/0000-0003-0993-7708
ORCID Icon,
Gaber El-Saber Batiha7 Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicines, Damanhour University, Damanhour, 22511, Egypt
,
Mohamed A El-Mokhtar8 Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, 71515, EgyptORCID Iconhttps://orcid.org/0000-0003-1943-8419
ORCID Icon &
Helal F Hetta8 Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt;9 Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USACorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0001-8541-7304
ORCID Icon show all
Pages 555-563 | Published online: 12 Feb 2021

Figures & data

Table 1 The Primers Used in the Current Study for PCR Amplification

Figure 1 Antibiotic resistance profile of K. pneumoniae.

Figure 1 Antibiotic resistance profile of K. pneumoniae.

Table 2 Efflux Activity at Varying Concentrations of Ethidium Bromide and Corresponding gyrA and parC Gene Mutations

Figure 2 Gel electrophoresis for gyrAparCaac(6)Ib, qepAqnrB, andqnrS genes.

Figure 2 Gel electrophoresis for gyrAparCaac(6)Ib, qepAqnrB, andqnrS genes.

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