A Comparative Study of Fluoroquinolone-Resistant Escherichia coli Lineages Portrays Indistinguishable Pathogenicity- and Survivability-Associated Phenotypic Characteristics Between ST1193 and ST131

Figures & data

Figure 1 Pathogenicity-associated characteristics of E. coli group ST1193 and group ST131. (A) Adhesion capability on T24 bladder epithelial cells. (B) Invasion capability on T24 bladder epithelial cells.

Notes: *p<0.05, **p<0.01 and ****p<0.0001 compared to negative control.

Abbreviations:E. coli, Escherichia coli; ST, sequence type.

Table 1 Prevalence of Biofilm Formation Among Different E. coli Isolates

ST Type	No. (%) of  E. coli Isolates with Biofilm Formation Ability
	Strong	Moderate	Weak	Absent
ST1193 (n=15)	1 (6.7%)	3 (20.0%)	10 (66.7%)	1 (6.7%)
ST131 (n=15)	2 (13.3%)	2 (13.3%)	11 (73.4%)	0 (0%)
E. faecalis ATCC 29212^a	1 (100.0%)	/	/	/
Medium alone (ODc)^b	/	/	/	1 (100.0%)

Notes: ^aPositive control; ^bNegative control.

Abbreviations: E. coli, Escherichia coli; ST, sequence type; E. faecalis, Enterococcus faecalis.

Figure 2 Macrocolonies of E. coli group ST1193 and group ST131. (A) 5-day-old macrocolonies of positive controls and negative control on macrocolony formation plates based on the presence and/or absence of curli and cellulose. (B) Curli and cellulose production distribution of E. coli ST1193 and ST131 isolates.

Abbreviations:E. coli, Escherichia coli; ST, sequence type.

Figure 3 Survivability-associated characteristics of E. coli group ST1193 and group ST131. (A) Resistance to serum bactericidal activity against 50% human serum. (B) Resistance to hydrogen peroxide at MIC level. (C) Resistance to hydrogen peroxide at MBC level. (D) Anti-phagocytic activity to RAW264.7 cells.

Notes: *p<0.05, ***p<0.01 and ****p<0.0001 compared to negative control.

Abbreviations:E. coli, Escherichia coli; ST, sequence type. MIC, minimum inhibitory concentration; MBC, minimum bactericidal concentration.